[Effect of tail-suspension on the reproduction of adult male rats].
[Effect of tail-suspension on the reproduction of adult male rats].
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发表时间：
2006-04
影响因子：
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通讯作者：
Dang-xia Zhou;S. Qiu;Zhi-Yong Wang;Jie Zhang
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作者：
Dang-xia Zhou;S. Qiu;Zhi-Yong Wang;Jie Zhang
目的研究尾部悬吊模拟失重对成年雄性大鼠生殖功能的影响及其机制，为探索太空环境对人类生殖功能的影响做基础性工作。方法40只SD雄性大鼠随机分为4组，实验组和对照组各2只。将两组大鼠分别尾吊14 d和28 d，观察实验组和对照组大鼠睾丸重量、形态、精子质量和数量，放射免疫法测定血清激素水平，TUNEL法检测睾丸细胞凋亡率。结果尾部悬吊后，睾丸重量、精子数和精子活动率均显著降低（P0.05）。两个实验组间差异无统计学意义（P > 0.05）。此外，实验组大鼠生精小管萎缩，生精上皮层数减少，曲细精管内精子数量减少。上述变化在28 d实验组更为显著。结论模拟失重对成年雄性大鼠生殖功能有不良影响。这些可能是通过诱导细胞凋亡引起的。阻断睾丸细胞的凋亡可能有助于改善这种不良影响。
OBJECTIVE To study the effects on the male reproduction in adult male rats and its mechanisms through simulated weightlessness using tail-suspension, in order to do a basic works of exploring the effects on human being's reproduction in outer space. METHODS Forty Spraque-Dawley adult male rats were randomly divided into four groups, two experimental groups and two control groups. Rats in the two experimental groups were tail-suspended for 14 d and 28 d respectively, then we examined the weight and morphology of testis, the quality and amount of sperm, also tested the serum hormone by radioimmunoassay and analyzed apoptosis rate of testicular cells by TUNEL in the experimental rats and control rats. RESULTS After tail-suspension, the weight of testis, the sperm count and sperm motility significantly decreased (P 0.05). These changes were not significant between two experimental groups (P > 0.05). In addition, the seminiferous tubules became atrophy with the reduction of the layers of seminiferous epithelium, and sperm amount in lumens of seminiferous tubules decreased in experimental groups. The above were more remarkable in the 28 d experimental group. CONCLUSION Simulating weightlessness has a harmful effect on reproduction of adult male rats. These may be caused by inducing apoptosis. The blocking apoptosis of testicular cells may be useful in improving the harmful effect.