Taxonomic study of polymorphic basidiomycetous fungi Sirobasidium and Sirotrema: Sirobasidium apiculatum sp. nov., Phaeotremella translucens comb. nov. and rediscovery of Sirobasidium japonicum in Japan
Taxonomic study of polymorphic basidiomycetous fungi Sirobasidium and Sirotrema: Sirobasidium apiculatum sp. nov., Phaeotremella translucens comb. nov. and rediscovery of Sirobasidium japonicum in Japan
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DOI：
10.1007/s10482-022-01787-9
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发表时间：
2022-11
期刊：
影响因子：
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通讯作者：
Muneki Yamada;Rikiya Endoh;Hiroshi Masumoto;Yuma Yoshihashi;M. Ohkuma;Yousuke Degawa
中科院分区：
文献类型：
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作者：
Muneki Yamada;Rikiya Endoh;Hiroshi Masumoto;Yuma Yoshihashi;M. Ohkuma;Yousuke Degawa
SirobasidiumandSirotrema属（银耳目、银耳菌纲、伞菌亚门、担子菌门）的物种仅根据有性型的形态学进行了描述，其中许多缺乏无性型酵母状态的分离株和核苷酸序列数据。从日本采集的担子中首次建立了Sirotrema japonicum和Sirobasidium japonicum菌株。此外，还分离到一个未描述的Sirobasidium属种。其特征是上担子具细尖，担子为二细胞，被纵隔膜分隔，这是该属中独特的特征组合。虽然Sb. japonicum在Tremellales中没有被解析，Sirobasidiumsp.与Sb形成了一个支持较好的单系分支。大型和纤维担子菌属，黑木耳属Sirotrema aciducens。单担孢子交配试验表明，Sb. japonicum在营养贫乏的培养基上产生担子、上担子和担子孢子，并且在受控条件下成功地完成了生活史。综上所述，我们提出Sirobasidium apiculatumsp. nov.和黑木耳Phaeotremella aciducenscomb. Nov.
Species in the generaSirobasidiumandSirotrema(Tremellales,Tremellomycetes,Agaricomycotina,Basidiomycota) have been described based solely on the morphology of teleomorph, and many of them lack both isolates of anamorphic yeast state and nucleotide sequence data. Strains ofSirotrema translucensandSirobasidium japonicumwere established for the first time from basidiocarps collected in Japan. Also, an undescribed species in the genusSirobasidiumwas isolated.Sirobasidiumsp. was characterized by its apiculate epibasidia and 2-celled basidia divided by a longitudinal septum, which is a unique combination of characteristics in the genus. Although the phylogenetic placement ofSb. japonicumwithin theTremellaleswas not resolved in our analysis,Sirobasidiumsp. formed a well-supported monophyletic clade withSb. magnumandFibulobasidiumspp., andSirotrema translucenswas located in the genusPhaeotremella. Mating experiments using single-basidiospore strains showed thatSb. japonicumproduced basidia, epibasidia, and basidiospores on a nutrient-poor medium, and the life cycle was successfully completed in controlled conditions. In conclusion, we proposeSirobasidium apiculatumsp. nov. andPhaeotremella translucenscomb. nov.