A Synergistic Transcriptional Regulation of Olfactory Genes Drives Blood-Feeding Associated Complex Behavioral Responses in the Mosquito Anopheles culicifacies
A Synergistic Transcriptional Regulation of Olfactory Genes Drives Blood-Feeding Associated Complex Behavioral Responses in the Mosquito Anopheles culicifacies
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DOI：
10.3389/fphys.2018.00577
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发表时间：
2018-05-23
影响因子：
4
通讯作者：
Dixit, Rajnikant
中科院分区：
文献类型：
--
作者：
Das De, Tanwee;Thomas, Tina;Dixit, Rajnikant
破解成年雌蚊寻找宿主和吸血行为进化/适应的分子基础，可能为设计新的分子策略来破坏人蚊相互作用提供机会。尽管在蚊子嗅觉和化学检测领域取得了很大的进展，但对成年雌蚊的特殊嗅觉系统的性别特异性进化知之甚少，该系统使它们能够驱动和管理复杂的吸血相关行为反应。血餐前后嗅觉系统的RNA-Seq综合分析。Culicifacies蚊子揭示了一个微小但独特的性质和关键嗅觉基因调控的变化，可能在管理各种行为反应中发挥关键作用。基于年龄依赖性转录谱分析，我们进一步证明了成年雌蚊的化学感觉系统在5-6天龄时逐渐学习和成熟，从而驱动寻宿主和吸血行为。气味结合蛋白（OBPs）的时间尺度表达分析揭示了与深夜至午夜叮咬高峰时间的独特关联。血餐诱导的独特OBP基因组和气味受体（Ors）表达的转换与蚊子先天生理状态的变化一致。血餐后续实验进一步提供了足够的证据，证明obps - or的协同和并发作用如何驱动“血餐前后”相关的复杂行为事件。两种感觉附属物蛋白（sap1和SAP2）在安腿中的显性表达。culicifacies的研究表明，这种蚊子可能有一个额外的优势，那就是拥有比安蚊更敏感的附属物。印度次大陆的城市疟疾病媒斯氏菌。最后，我们的分子模型分析预测了未来感觉附属物蛋白功能表征的关键氨基酸残基，这些氨基酸残基可能在调节An的多种行为中发挥核心作用。culicifacies蚊子。意义吸血行为的进化和适应不仅有利于雌蚊繁殖成功，而且使其成为重要的疾病传播媒介。出现后的环境暴露可能有利于蚊子广泛调节的嗅觉系统，以驱动复杂的行为反应。但是，这些嗅觉衍生的遗传因素如何管理女性特定的“前后”血餐相关的复杂行为反应尚不清楚。我们的研究结果表明，嗅觉因素的协同作用可能控制着先天的学习策略，以促进快速的血食获取和下游行为活动。一项物种特异性转录谱分析和计算机分析预测，“感觉附属物蛋白”可能是设计针对culicifacies按蚊的定向迷失策略的独特靶点。
Decoding the molecular basis of host seeking and blood feeding behavioral evolution/adaptation in the adult female mosquitoes may provide an opportunity to design new molecular strategy to disrupt human-mosquito interactions. Although there is a great progress in the field of mosquito olfaction and chemo-detection, little is known about the sex-specific evolution of the specialized olfactory system of adult female mosquitoes that enables them to drive and manage the complex blood-feeding associated behavioral responses. A comprehensive RNA-Seq analysis of prior and post blood meal olfactory system of An. culicifacies mosquito revealed a minor but unique change in the nature and regulation of key olfactory genes that may play a pivotal role in managing diverse behavioral responses. Based on age-dependent transcriptional profiling, we further demonstrated that adult female mosquito's chemosensory system gradually learned and matured to drive the host-seeking and blood feeding behavior at the age of 5-6 days. A time scale expression analysis of Odorant Binding Proteins (OBPs) unravels unique association with a late evening to midnight peak biting time. Blood meal-induced switching of unique sets of OBP genes and Odorant Receptors (Ors) expression coincides with the change in the innate physiological status of the mosquitoes. Blood meal follows up experiments further provide enough evidence that how a synergistic and concurrent action of OBPs-Ors may drive "prior and post blood meal" associated complex behavioral events. A dominant expression of two sensory appendages proteins (SAP-1 & SAP2) in the legs of An. culicifacies suggests that this mosquito species may draw an extra advantage of having more sensitive appendages than An. stephensi, an urban malarial vector in the Indian subcontinents. Finally, our molecular modeling analysis predicts crucial amino acid residues for future functional characterization of the sensory appendages proteins which may play a central role in regulating multiple behaviors of An. culicifacies mosquito.SIGNIFICANCEEvolution and adaptation of blood feeding behavior not only favored the reproductive success of adult female mosquitoes but also make them important disease-transmitting vectors. An environmental exposure after emergence may favor the broadly tuned olfactory system of mosquitoes to drive complex behavioral responses. But, how these olfactory derived genetic factors manage female specific "pre and post" blood meal associated complex behavioral responses are not well known. Our findings suggest that a synergistic action of olfactory factors may govern an innate to prime learning strategy to facilitate rapid blood meal acquisition and downstream behavioral activities. A species-specific transcriptional profiling and an in-silico analysis predict that "sensory appendages protein" may be a unique target to design disorientation strategy against the mosquito Anopheles culicifacies.