Surface-induced dissociation shows potential to be more informative than collision-induced dissociation for structural studies of large systems.
Surface-induced dissociation shows potential to be more informative than collision-induced dissociation for structural studies of large systems.
复制标题
对于大型系统的结构研究，表面诱导解离显示出比碰撞诱导解离提供更多信息的潜力。
DOI：
10.1016/j.jasms.2008.04.026
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发表时间：
2008
影响因子：
3.2
通讯作者：
Blackwell,AnneE
中科院分区：
文献类型：
--
作者：
Wysocki,VickiH;Jones,ChristopherM;Galhena,AsiriS;Blackwell,AnneE
在气相中保持非共价大分子组装体完整的能力为质谱法表征尺寸不断增加的离子铺平了道路，并成为结构生物学领域的有力工具。串联质谱实验有可能通过大分子复合物的气相解离来扩展该技术的能力，但是与小气体原子的碰撞目前提供非常有限的碎片化。解离大离子的另一种方法是将它们碰撞到一个表面，一个更大的目标。在这里，我们展示了通过表面诱导解离（SID）使大蛋白质复合物和无机盐簇片段化的能力和益处，所述表面诱导解离提供了这些系统的更广泛的片段化，并显示出作为用于增加大小的离子的活化方法的前景。
The ability to preserve noncovalent, macromolecular assemblies intact in the gas phase has paved the way for mass spectrometry to characterize ions of increasing size and become a powerful tool in the field of structural biology. Tandem mass spectrometry experiments have the potential to expand the capabilities of this technique through the gas-phase dissociation of macromolecular complexes, but collisions with small gas atoms currently provide very limited fragmentation. One alternative for dissociating large ions is to collide them into a surface, a more massive target. Here, we demonstrate the ability and benefit of fragmenting large protein complexes and inorganic salt clusters by surface-induced dissociation (SID), which provides more extensive fragmentation of these systems and shows promise as an activation method for ions of increasing size.