Pod shattering resistance associated with domestication is mediated by a NAC gene in soybean
Pod shattering resistance associated with domestication is mediated by a NAC gene in soybean
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与驯化相关的破荚抗性是由大豆中的 NAC 基因介导的
DOI：
10.1038/ncomms4352
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发表时间：
2014-02-01
影响因子：
16.6
通讯作者：
Wang, Yin-Zheng
中科院分区：
文献类型：
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作者：
Dong, Yang;Yang, Xia;Wang, Yin-Zheng
种子传播能力的丧失是古代人类选择的一个关键农艺性状，被视为作物驯化的里程碑。在这项研究中，在豆科作物大豆中，Glycine max(L.) Merr。为人类提供植物油和蛋白质，我们发现抗破碎性状的关键细胞特征在于过度木质化的纤维帽细胞（FCC），而荚果腹缝中的离层未发生变化。我们证明，NAC（NAM、ATAF1/2 和 CUC2）基因 SHATTERING1-5（SHAT1-5）可功能性激活次生壁生物合成，并通过以野生等位基因水平 15 倍的表达促进 FCC 次生壁显着增厚，这归因于上游阻遏蛋白的功能破坏。我们表明，SHAT1-5 的强人工选择已导致对 16 号染色体上约 116 kb 的严重选择性扫描。这种基因座和调节机制可适用于豆科作物改良。
Loss of seed dispersal is a key agronomical trait targeted by ancient human selection and has been regarded as a milestone of crop domestication. In this study, in the legume crop soybeanGlycine max(L.) Merr. which provides vegetable oils and proteins for humans, we show that the key cellular feature of the shattering-resistant trait lies in the excessively lignified fibre cap cells (FCC) with the abscission layer unchanged in the pod ventral suture. We demonstrate that a NAC (NAM, ATAF1/2 and CUC2) geneSHATTERING1-5(SHAT1-5) functionally activates secondary wall biosynthesis and promotes the significant thickening of FCC secondary walls by expression at 15-fold the level of the wild allele, which is attributed to functional disruption of the upstream repressor. We show that strong artificial selection ofSHAT1-5has caused a severe selective sweep across ~116 kb on chromosome 16. This locus and regulation mechanism could be applicable to legume crop improvement.