THE HUMAN MET ONCOGENE IS RELATED TO THE TYROSINE KINASE ONCOGENES
THE HUMAN MET ONCOGENE IS RELATED TO THE TYROSINE KINASE ONCOGENES
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DOI：
10.1038/318385a0
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发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
VANDEWOUDE, GF
中科院分区：
文献类型：
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作者：
DEAN, M;PARK, M;VANDEWOUDE, GF
Themetoncogene 之前是从化学转化的人类细胞系 MNNG-HOS 中分离出来的（参考文献 1、2）。最近的证据表明，原癌基因座表达两类转录本。然而，Themetoncogene 表达一种异常的 RNA，其序列与两个转录物具有共同的序列。我们现在报告了人类致癌基因的部分核苷酸测序，并表明该基因与蛋白激酶癌基因和生长因子受体相关。异核苷酸序列与任何已发表的基因的序列不相同，并且它与酪氨酸激酶比与丝氨酸/苏氨酸激酶更接近同源。在酪氨酸激酶家族中，测序的met结构域与人胰岛素受体和病毒abl基因关系最为密切。原位染色体杂交已将met结构域定位到人7号染色体条带7q21-q31，该位置与其他激酶不同。这也是与在部分急性非淋巴细胞白血病患者中观察到的非随机染色体缺失相关的区域。随附的论文3表明，该染色体位点也与人类遗传性疾病囊性纤维化密切相关。
Themetoncogene was previously isolated from a chemically transformed human cell line, MNNG-HOS (refs 1, 2). Recent evidence has demonstrated that two classes of transcripts are expressed from themetproto-oncogene locus. Themetoncogene, however, expresses an aberrant RNA which has sequences in common with both transcripts. We now report partial nucleotide sequencing of the humanmetoncogene and show thatmetis related to the protein kinase oncogenes and growth factor receptors. Themetnucleotide sequence is not identical to that of any published gene, and it is more closely homologous to the tyrosine kinases than to the serine/threonine kinases. Within the tyrosine kinase family, the sequencedmetdomains are most closely related to the human insulin receptor and the viralablgene.In situchromosome hybridization has mappedmetto human chromosome 7 band 7q21–q31, a location distinct from that of other kinases. This is also a region associated with nonrandom chromosomal deletions observed in a portion of patients with acute non-lymphocytic leukaemia. The accompanying paper3shows that this chromosomal locus is also tightly linked with the human heredity disease cystic fibrosis.