Reproductive Biology and Susceptibility of Florida Culex coronator to Infection with West Nile Virus
Reproductive Biology and Susceptibility of Florida Culex coronator to Infection with West Nile Virus
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DOI：
10.1089/vbz.2013.1501
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发表时间：
2014-08-01
影响因子：
2.1
通讯作者：
Karr, Nicholas
中科院分区：
文献类型：
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作者：
Alto, Barry W.;Connelly, C. Roxanne;Karr, Nicholas
嗜鸟库蚊被认为是西尼罗病毒（WNV）在鸟类宿主中的主要扩增媒介，也是造成流行病传播的媒介。冠状库蚊于2005年首次在佛罗里达州的奥卡卢萨县、圣罗莎县、沃尔顿县和华盛顿县采集，此后蔓延到整个州。Cx的向量能力。西尼罗河病毒的冠状病毒，已知在本质上是受感染的，尚未得到评估。如果没有这方面的知识，我们就无法评估该物种在佛罗里达州作为西尼罗河病毒地方性和流行媒介的潜力。在本研究中，我们研究了西尼罗河病毒感染、传播和传播的生殖生物学和易感性。coronator。我们证明了Cx。Coronator能够在吸血后延迟数周产卵，并且鸟类的产卵数量比哺乳动物宿主要多。残雪。冠状体极易感染（接近80-100%）和传播（接触后18天接近65-85%），传播率较低（25℃时为0-17%，28℃时为28-67%），表明在某些条件下它是西尼罗河病毒的有效载体。传播感染的比例与暴露时间有关，28℃时比25℃时高。整个佛罗里达的验尸官构成了潜在的公共健康风险这一基线知识对于蚊虫控制和公共卫生机构评估美国东南部当前和未来的疾病风险至关重要。
Ornithophilic Culex species are considered the primary amplification vectors of West Nile virus (WNV) in bird hosts as well as vectors responsible for epidemic transmission. Culex coronator was first collected from Okaloosa, Santa Rosa, Walton, and Washington Counties in Florida in 2005 and has since spread throughout the state. The vector competence of Cx. coronator for WNV, known to be infected in nature, has not been assessed. Without this knowledge, we are unable to assess this species' potential as an enzootic and epidemic vector of WNV in Florida. In the current study, we investigate the reproductive biology and susceptibility to WNV infection, dissemination, and transmission by Cx. coronator. We show that Cx. coronator is capable of delaying oviposition for several weeks after blood feeding and that the number of eggs laid is greater for avian than mammalian hosts. Cx. coronator were highly susceptible to infection (similar to 80-100%) and dissemination (similar to 65-85% by 18 days since exposure) with lower rates of transmission (0-17% at 25 degrees C and 28-67% at 28 degrees C), suggesting that it is a competent vector of WNV under some conditions. The proportion of mosquitoes with disseminated infections related to the time since exposure and was higher at 28 degrees C than at 25 degrees C. The rapid and statewide distribution of Cx. coronator throughout Florida poses as a potential public health risk. This baseline knowledge is essential information for mosquito control and public health agencies to assess current and future disease risk to Southeastern United States.