Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8α molecule
Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8α molecule
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DOI：
10.1093/ps/78.11.1526
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发表时间：
1999-11-01
期刊：
影响因子：
4.4
通讯作者：
Vainio, O
中科院分区：
文献类型：
--
作者：
Li, Z;Nestor, KE;Vainio, O
为了测量火鸡CD 4和CD 8 T细胞水平，通过流式细胞术分析从五个火鸡系中的个体获得的血液来测试小鼠抗鸡CD 4和CD 8单克隆抗体（mAb）与火鸡白细胞的交叉反应性。使用的火鸡品系包括随机繁殖的对照群体（RBC 2）、选择用于增加16周龄BW的RBC 2的亚系（F）和来自三个商业火鸡育种者中的每一个的父系（A、B和C）。分离外周血淋巴细胞，用单色或双色染色。CT 8 mAb（抗鸡CD 8 α）在某些火鸡中未能检测到CD 8 α分子，并且检测CD 8 α分子的能力存在较大的品系差异。然而，某些抗鸡CD 8 α mAb（3-298、3-292和11-39）与火鸡CD 8 α分子具有良好的交叉反应性。这些现有数据表明火鸡CD 8 α：分子是多态性的。一些抗鸡CD 4 mAb（CT 4、2-6、2-35和7-125）也与火鸡CD 4分子交叉反应。免疫沉淀和Western印迹显示，3-298 mAb在还原条件下从火鸡脾细胞裂解物中沉淀出33- 35-kDa的多肽。交叉反应性抗鸡CD 4和CD 8单克隆抗体的获得将促进火鸡免疫应答的研究。
To measure turkey CD4 and CD8 T cell levels, the cross-reactivity of mouse anti-chicken CD4 and CD8 monoclonal antibodies (mAb) with turkey leucocytes was tested by flow cytometric analysis of blood obtained from individuals in five turkey lines. The turkey lines used included a randombred control population (RBC2), a subline (F) of RBC2 selected for increased 16-wk BW, and a sire line (A, B, and C)from each of three commercial turkey breeders. Peripheral blood lymphocytes were isolated and stained with single or dual color staining. The CT8 mAb (anti-chicken CD8 alpha) failed to detect the CD8 alpha molecule in some turkeys, and there were large line differences in ability to detect the CD8 alpha molecule. However, certain anti-chicken CD8 alpha mAb (3-298, 3-292 and 11-39) had good cross-reactivity with the turkey CD8 alpha molecule. These present data indicate that the turkey CD8 alpha: molecule is polymorphic. Some anti-chicken CD4 mAb (CT4, 2-6, 2-35, and 7-125) were also crossreactive with the turkey CD4 molecule. Immunoprecipitation and Western blotting showed that the 3-298 mAb precipitated a 33- to 35-kDa polypeptide from the turkey splenocyte lysate under reducing conditions. The availability of cross-reactive anti-chicken CD4 and CD8 mAb will facilitate the studies of immune responses in turkeys.