Grhl2 is Required in Nonneural Tissues for Neural Progenitor Survival and Forebrain Development
Grhl2 is Required in Nonneural Tissues for Neural Progenitor Survival and Forebrain Development
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DOI：
10.1002/dvg.22875
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发表时间：
2015-09-01
期刊：
影响因子：
1.5
通讯作者：
Stottmann, Rolf W.
中科院分区：
文献类型：
--
作者：
Menke, Chelsea;Cionni, Megan;Stottmann, Rolf W.
颗粒状基因是一个高度保守的基因家族的一部分，在哺乳动物外胚层的发育和维持中起着许多作用。在这里，我们在从器官发生缺陷的ENU突变筛选中恢复的小鼠系中发现了Grhl2的一个新的等位基因，cleft-face 3 （clft3）。纯合子clft3突变体与Grhl2的其他等位基因具有许多共同的表型。我们注意到遗传背景对clft3表型的显著影响。其中之一是端脑的大小减少，我们在突变的大脑中发现神经祖细胞有丝分裂和细胞凋亡的异常模式。有趣的是，Grhl2在发育中的前脑中不表达，这表明这是神经祖细胞的一种生存因子，对来自Grhl2高表达的外胚层的神经组织发挥旁分泌作用。(C) 2015 Wiley期刊公司
Grainyhead-like genes are part of a highly conserved gene family that play a number of roles in ectoderm development and maintenance in mammals. Here we identify a novel allele of Grhl2, cleft-face 3 (clft3), in a mouse line recovered from an ENU mutagenesis screen for organogenesis defects. Homozygous clft3 mutants have a number of phenotypes in common with other alleles of Grhl2. We note a significant effect of genetic background on the clft3 phenotype. One of these is a reduction in size of the telencephalon where we find abnormal patterns of neural progenitor mitosis and apoptosis in mutant brains. Interestingly, Grhl2 is not expressed in the developing forebrain, suggesting this is a survival factor for neural progenitors exerting a paracrine effect on the neural tissue from the overlying ectoderm where Grhl2 is highly expressed. (C) 2015 Wiley Periodicals, Inc.