Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development
Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development
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DOI：
10.1126/science.1068768
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发表时间：
2002-04-05
期刊：
影响因子：
56.9
通讯作者：
Singh, H
中科院分区：
文献类型：
--
作者：
Kosak, ST;Skok, JA;Singh, H
免疫球蛋白(Ig)基因座在B淋巴细胞发育过程中被选择性地激活以进行转录和重排。通过荧光原位杂交，我们发现Ig Heavy(H)和Ig kappa基因座在造血祖细胞和前T细胞中优先定位在核周，但在前B核中集中配置。外围的非活性位点与着丝粒异染色质无关。在原-B细胞中，当远离核周时，IgH位点似乎经历了大规模的压缩。我们认为，亚核定位是调节淋巴细胞发育过程中IgH和Igkappa基因转录和重组的一种新方法。
Immunoglobulin (Ig) loci are selectively activated for transcription and rearrangement during B lymphocyte development. Using fluorescence in situ hybridization, we show that Ig heavy (H) and Igkappa Loci are preferentially positioned at the nuclear periphery in hematopoietic progenitors and pro-T cells but are centrally configured in pro-B nuclei. The inactive loci at the periphery do not associate with centromeric heterochromatin. Upon localization away from the nuclear periphery in pro-B cells, the IgH locus appears to undergo large-scale compaction. We suggest that subnuclear positioning represents a novel means of regulating transcription and recombination of IgH and Igkappa loci during lymphocyte development.