Sesamol: a natural phenolic compound with promising anticandidal potential.
Sesamol: a natural phenolic compound with promising anticandidal potential.
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DOI：
10.1155/2014/895193
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发表时间：
2014
影响因子：
2.6
通讯作者：
Hameed S
中科院分区：
文献类型：
--
作者：
Ansari MA;Fatima Z;Hameed S
我们研究了芝麻酚（sesamol，一种天然酚类化合物）的抗真菌作用，并举例说明它可能通过破坏白色念珠菌（一种人类真菌病原体）的钙调磷酸酶信号通路来介导。其抗真菌活性不仅限于白色念珠菌及其6个临床分离株，而且对非白色念珠菌也有抑制作用。有趣的是，Ses的抗真菌作用既不影响MDR外排转运体活性，也不影响药物的被动扩散。我们发现，Ses处理的白色念珠菌复制了钙调磷酸酶信号缺陷细胞所显示的表型，导致对碱性pH、离子、膜、盐度、内质网和血清应激敏感，但对热应激仍具有抗性。此外，麦角甾醇水平显著降低了63%，证实了通过透射电子显微镜观察到的膜扰动对Ses的响应。尽管事实上Ses处理模拟钙调磷酸酶信号受损的表型，但它独立于细胞壁完整性途径，如斑点试验和扫描电子显微照片所示。综上所述，从本研究中获得的数据清楚地确定了Ses是一种有效的抗真菌剂，可以胜任地用于治疗念珠菌感染。
We investigated the antifungal effects of sesamol (Ses), a natural phenolic compound, and exemplified that it could be mediated through disruption of calcineurin signaling pathway in C. albicans, a human fungal pathogen. The repertoire of antifungal activity not only was limited to C. albicans and its six clinical isolates tested but also was against non-albicans species of Candida. Interestingly, the antifungal effect of Ses affects neither the MDR efflux transporter activity nor passive diffusion of drug. We found that C. albicans treated with Ses copies the phenotype displayed by cells having defect in calcineurin signaling leading to sensitivity against alkaline pH, ionic, membrane, salinity, endoplasmic reticulum, and serum stresses but remained resistant to thermal stress. Furthermore, the ergosterol levels were significantly decreased by 63% confirming membrane perturbations in response to Ses as also visualized through transmission electron micrographs. Despite the fact that Ses treatment mimics the phenotype of compromised calcineurin signaling, it was independent of cell wall integrity pathway as revealed by spot assays and the scanning electron micrographs. Taken together, the data procured from this study clearly ascertains that Ses is an effectual antifungal agent that could be competently employed in treating Candida infections.