The Effect of Iatrogenic Staphylococcus epidermidis Intercellar Adhesion Operon on the Formation of Bacterial Biofilm on Polyvinyl Chloride Surfaces
The Effect of Iatrogenic Staphylococcus epidermidis Intercellar Adhesion Operon on the Formation of Bacterial Biofilm on Polyvinyl Chloride Surfaces
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DOI：
10.1089/sur.2013.129
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发表时间：
2014-12-01
影响因子：
2
通讯作者：
Guo Fengli
中科院分区：
文献类型：
--
作者：
Ye Lianhua;Huang Yunchao;Guo Fengli
背景：表皮葡萄球菌的细胞间粘附基因（ica）是细菌聚集的关键因素。本研究探讨了 ica 对聚氯乙烯 (PVC) 表面细菌生物膜形成的影响。方法：从 112 个医源性表皮葡萄球菌临床分离株中鉴定并测序与细菌生物膜形成相关的基因，包括 16S rRNA、自溶素 (atlE)、纤维蛋白原结合蛋白基因 (fbe) 和 ica。 聚合酶链反应（PCR）和基因测序。根据测序结果，分离ica操纵子阳性菌株（icaADB+/atlE+/fbe+）和ica操纵子阴性菌株（icaADB-/atlE+/fbe+），并与PVC材料共培养。共培养6、12、18、24、30 h后，在共聚焦激光扫描显微镜和扫描电镜下测量PVC表面细菌生物膜厚度和菌落数量。结果：112株医源性菌株中表皮葡萄球菌特异性16SrRNA阳性率为100%（112/112）。 ica阳性（icaADB+/atlE+/fbe+）基因型菌株占57.1%（64/112），ica阴性（icaADB-/atlE+/fbe+）基因型菌株占37.5%（42/112）。共培养30 h，ica阳性组PVC表面未形成明显的细菌生物膜，但共培养24 h后，形成成熟的细菌生物膜结构。在所有时间点，ica操纵子阳性组的PVC表面细菌生物膜厚度和细菌菌落数量均显着高于ica操纵子阴性组（p
Background: The intercellular adhesion gene (ica) of Staphylococcus epidermidis is a key factor for bacterial aggregation. This study explored the effect of ica on the formation of bacterial biofilm on polyvinyl chloride (PVC) surfaces.Methods: Genes related to bacterial biofilm formation, including 16S rRNA, autolysin (atlE), fibrinogen binding protein gene (fbe), and ica were identified and sequenced from 112 clinical isolates of iatrogenic S. epidermidis by polymerase chain reaction (PCR) and gene sequencing. Based on the sequencing result, ica operon-positive (icaADB+/atlE+/fbe+) and ica operon-negative (icaADB-/atlE+/fbe+) strains were separated and co-cultivated with PVC material. After 6, 12, 18, 24, and 30 h of co-culture, the thickness of the bacterial biofilm and quantity of bacterial colony on the PVC surface were measured under the confocal laser scanning microscope and scanning electron microscope.Results: The positive rate of S. epidermidis-specific 16SrRNA in 112 iatrogenic strains was 100% (112/112). The genotype of ica-positive (icaADB+/atlE+/fbe+) strains accounted for 57.1% (64/112), and genotype of ica-negative (icaADB-/atlE+/fbe+) strains accounted for 37.5% (42/112). During 30 h of co-culture, no obvious bacterial biofilm formed on the surface of PVC in the ica-positive group, however, mature bacterial biofilm structure formed after 24 h. For all time points, thickness of bacterial biofilm and quantity of bacterial colony on PVC surfaces in the ica operon-positive group were significantly higher than those in ica operon-negative group (p