Multiple strategies for translesion synthesis in bacteria.
Multiple strategies for translesion synthesis in bacteria.
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DOI：
10.3390/cells1040799
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发表时间：
2012-10-15
期刊：
影响因子：
6
通讯作者：
Beuning PJ
中科院分区：
文献类型：
--
作者：
Ippoliti PJ;Delateur NA;Jones KM;Beuning PJ
DNA 损伤很常见，可能由多种环境和内源引起。为了应对普遍存在的 DNA 损伤，Y 家族 DNA 聚合酶由 SOS 反应诱导，能够绕过 DNA 损伤。在大肠杆菌中，这些 Y 家族聚合酶是 DinB 和 UmuC，其活性通过与聚合酶管理蛋白 UmuD 的相互作用来调节。许多（但不是全部）细菌利用 DinB 和 UmuC 同源物。最近，人们发现一种名为 ImuC 的 C 家族聚合酶，其一级结构与复制 DNA 聚合酶 DnaE 相似，能够复制受损的 DNA，并执行或抑制诱变。 ImuC 通常与蛋白质 ImuA 和 ImuB 一起发现，后者与 Y 家族聚合酶相似，但似乎缺乏聚合酶活性所需的催化残基。该 imuAimuBimuC 诱变盒代表了细菌跨损伤合成和诱变的广泛替代策略。细菌 Y 家族和 ImuC DNA 聚合酶有助于复制 DNA 损伤并获得抗生素耐药性。
Damage to DNA is common and can arise from numerous environmental and endogenous sources. In response to ubiquitous DNA damage, Y-family DNA polymerases are induced by the SOS response and are capable of bypassing DNA lesions. In Escherichia coli, these Y-family polymerases are DinB and UmuC, whose activities are modulated by their interaction with the polymerase manager protein UmuD. Many, but not all, bacteria utilize DinB and UmuC homologs. Recently, a C-family polymerase named ImuC, which is similar in primary structure to the replicative DNA polymerase DnaE, was found to be able to copy damaged DNA and either carry out or suppress mutagenesis. ImuC is often found with proteins ImuA and ImuB, the latter of which is similar to Y‑family polymerases, but seems to lack the catalytic residues necessary for polymerase activity. This imuAimuBimuC mutagenesis cassette represents a widespread alternative strategy for translesion synthesis and mutagenesis in bacteria. Bacterial Y‑family and ImuC DNA polymerases contribute to replication past DNA damage and the acquisition of antibiotic resistance.