PTEN is a mechanosensing signal transducer for myosin II localization in Dictyostelium cells
PTEN is a mechanosensing signal transducer for myosin II localization in Dictyostelium cells
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DOI：
10.1111/j.1365-2443.2009.01312.x
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发表时间：
2009-07-01
期刊：
影响因子：
2.1
通讯作者：
Yumura, Shigehiko
中科院分区：
文献类型：
--
作者：
Pramanik, Md. Kamruzzaman;Iijima, Miho;Yumura, Shigehiko
为了研究PTEN在调节皮质运动活动中的作用，特别是在肌球蛋白II定位中，eGFP-PTEN和mRFP-myosin II在网骨细胞中同时表达。PTEN和肌球蛋白II共定位于迁移细胞的后部和分裂细胞的沟区。在悬浮培养中，PTEN敲除（pten（-））细胞变成多核的，肌球蛋白II的量显着减少在犁沟。在伪足收缩和微毛细管细胞抽吸过程中，PTEN先于肌球蛋白II在伪足尖端和抽吸的叶中积累。在pten（-）细胞中，只有少量的肌球蛋白II聚集在收缩的伪足和吸出的细胞叶。即使在肌球蛋白II无效的细胞和latrunculin B处理的细胞中，PTEN也在收缩伪足和吸出的裂片处积累，尽管量减少，这表明PTEN积累部分依赖于肌球蛋白II和皮质肌动蛋白。在肌球蛋白II之前的PTEN积累表明，PTEN是定位肌球蛋白II的信号通路中的上游组分，可能具有机械传感信号回路，其中肌动球蛋白驱动的收缩通过正反馈机制进一步增加PTEN和肌球蛋白II的积累。
To investigate the role of PTEN in regulation of cortical motile activity, especially in myosin II localization, eGFP-PTEN and mRFP-myosin II were simultaneously expressed in Dictyostelium cells. PTEN and myosin II co-localized at the posterior of migrating cells and furrow region of dividing cells. In suspension culture, PTEN knockout (pten(-)) cells became multinucleated, and myosin II significantly decreased in amount at the furrow. During pseudopod retraction and cell aspiration by microcapillary, PTEN accumulated at the tips of pseudopods and aspirated lobes prior to the accumulation of myosin II. In pten(-) cells, only a small amount of myosin II accumulated at the retracting pseudopods and aspirated cell lobes. PTEN accumulated at the retracting pseudopods and aspirated lobes even in myosin II null cells and latrunculin B-treated cells though in reduced amounts, indicating that PTEN accumulates partially depending on myosin II and cortical actin. Accumulation of PTEN prior to myosin II suggests that PTEN is an upstream component in signaling pathway to localize myosin II, possibly with mechanosensing signaling loop where actomyosin-driven contraction further augments accumulation of PTEN and myosin II by a positive feedback mechanism.