Identification of a nonchordate‐type classic cadherin in vertebrates: Chicken Hz‐cadherin is expressed in horizontal cells of the neural retina and contains a nonchordate‐specific domain complex
Identification of a nonchordate‐type classic cadherin in vertebrates: Chicken Hz‐cadherin is expressed in horizontal cells of the neural retina and contains a nonchordate‐specific domain complex
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脊椎动物中非脊索动物型经典钙粘蛋白的鉴定：鸡 Hz-钙粘蛋白在神经视网膜的水平细胞中表达，并包含非脊索动物特异性结构域复合物
作者：
K. Tanabe;M. Takeichi;S. Nakagawa
经典钙粘附素在多种动物中介导钙依赖的细胞-细胞黏附，但它们的结构域结构在脊索动物和非脊索动物之间存在显著差异。脊索型经典钙粘附素的胞外区(I型和II型经典钙粘附素)由5个串联的保守序列组成，称为EC结构域，而非脊索型经典钙粘附素的胞外区(称为III型经典钙粘附素)包含数量可变的EC结构域，紧随其后的是由层粘连蛋白-A球状结构域和EGF样重复序列组成的特征结构域复合体。在本研究中，我们发现了一种与果蝇N-钙粘蛋白序列高度相似的新的脊椎动物III型钙粘蛋白，并将其命名为鸡赫兹-钙粘蛋白(Chz-cadherin)，因为该分子在神经视网膜的水平细胞中有不同的表达。当Chz-钙粘附素被引入培养的细胞中时，它起到了黏附分子的作用。数据库搜索发现在斑马鱼中有一个Chz-钙粘素同源物，在河豚中有两个同源物，但在其他脊椎动物物种中没有。这些观察表明，III型经典钙粘附素在脊椎动物中是保守的，仅在有限的细胞类型中表达，但在脊椎动物的特定系统发育类群中丢失。《发展动力学》229：899-906,2004。©2004 Wiley-Liss Inc.
Classic cadherins mediate calcium‐dependent cell–cell adhesion in a variety of animals, but there are marked differences in their domain structures between chordate and nonchordate animals. The extracellular domain of chordate‐type classic cadherins (type I and type II classic cadherins) consists of five tandem repeats of conserved sequences called EC domains, whereas that of nonchordate‐type classic cadherins (designated as type III classic cadherin) contains a variable number of EC domains, followed by a characteristic domain complex made of laminin‐A globular domains and EGF‐like repeats. In the present study, we identified a novel vertebrate type III cadherin showing high sequence similarity to Drosophila N‐cadherin, and named this molecule chicken Hz‐cadherin (cHz‐cadherin), because of the distinct expression in horizontal cells of the neural retina. cHz‐cadherin functioned as an adhesion molecule when introduced into cultured cells. Database search revealed one cHz‐cadherin homologue in zebrafish and two in puffer fish, but none in other vertebrate species examined. These observations indicate that type III classic cadherins have been conserved in vertebrate species, being expressed by limited cells types, but lost in particular phylogenic groups of the vertebrates. Developmental Dynamics 229:899–906, 2004. © 2004 Wiley‐Liss, Inc.
影响因子：
4.4
作者：
Ten Belecky-Adams;Stanislav I. Tomarev;Hua-Shun;Li;Lynda Ploder;Roderick R. Mdnnes;OlofSundin;Ruben Adler
通讯作者：
Ten Belecky-Adams;Stanislav I. Tomarev;Hua-Shun;Li;Lynda Ploder;Roderick R. Mdnnes;OlofSundin;Ruben Adler