The potential linkage between antibiotic resistance genes and microbial functions across soil–plant systems
The potential linkage between antibiotic resistance genes and microbial functions across soil–plant systems
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DOI：
10.1007/s11104-023-06247-5
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发表时间：
2023-08
期刊：
影响因子：
4.9
通讯作者：
E. Xiao;Weimin Sun;Jinmei Deng;Li Shao;Zengping Ning;T. Xiao
中科院分区：
文献类型：
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作者：
E. Xiao;Weimin Sun;Jinmei Deng;Li Shao;Zengping Ning;T. Xiao
抗生素耐药基因（ARG）的增殖是全球范围内日益严重的公共卫生问题。一些研究已经证实了植物微生物组在促进抗生素耐药性传播方面发挥的重要作用，从而对人类健康构成重大威胁。然而，抗生素抗性从土壤到植物的传递的潜在机制在很大程度上仍然没有被探索。以东南景天为模式植物，研究了土壤-植物界面ARGs的分布和分布。的ARGs显着转移之间的根际和散装土壤，并链接到不同的变化，在整个土壤-植物界面的细菌功能。具体而言，富集在根际的ARGs显着相关的微生物功能的信号途径和降解途径。相反，根际ARGs的耗竭与微生物生物合成途径、养分循环和ABC运输系统的功能显著相关。此外，我们的调查涉及宏基因组重叠群组装，提供令人信服的证据表明，这些上述关系确实检测到组装bins.ConclusionsThe我们的研究结果揭示了ARGs的分布和细菌功能的变化之间的明确关联归因于根际微生物组在土壤-植物界面的组装。这些研究结果显着有助于提高我们的理解机制的ARGs从散装土壤根际土壤的传输。
AimsThe proliferation of antibiotic resistance genes (ARGs) is a growing public health concern worldwide. Several studies have substantiated the significant role played by the plant microbiome in facilitating the transmission of antibiotic resistance, thereby posing a substantial threat to human health. However, the underlying mechanism of the transmission of antibiotic resistance from soil to plants is still largely unexplored.MethodsIn the current study, we selectedSedum alfrediias a model plant to investigate the occurrence and distribution of ARGs across the soil–plant interface.ResultsOur results showed that 20.34% of ARGs were significantly shifted between rhizosphere and bulk soils and were linked to distinct variations in bacterial functions across the soil-plant interface. Specifically, the ARGs enriched in the rhizosphere were significantly correlated to microbial functions of signaling pathways and degradation pathways. Conversely, the ARGs depleted in rhizosphere were significantly correlated to microbial functions of biosynthesis pathways, nutrient cycling, and ABC transport system. Moreover, our investigation involved metagenomic contig assembly, providing compelling evidence that these aforementioned relationships were indeed detected in the assembled bins.ConclusionsThe results of our study revealed a clear association between the distribution of ARGs and changes in bacterial functions attributable to the assemblage of the rhizosphere microbiome at the soil-plant interface. These findings significantly contribute to enhancing our comprehension of the mechanism underlying the transmission of ARGs from bulk soils to rhizosphere soils.