Interleukin 16 expression and phenotype of interleukin 16 producing cells in Crohn's disease
Interleukin 16 expression and phenotype of interleukin 16 producing cells in Crohn's disease
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DOI：
10.1136/gut.49.6.795
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发表时间：
2001-12-01
期刊：
影响因子：
24.5
通讯作者：
Radzun, HJ
中科院分区：
文献类型：
--
作者：
Middel, P;Reich, K;Radzun, HJ
背景：克罗恩病发生和维持的机制尚不清楚。先前的研究表明，在克罗恩病炎症影响的肠壁内浸润的CD4+ T细胞数量增加。使用抗cd4抗体的新治疗方法被认为对克罗恩病有效。白细胞介素16 （IL-16）被认为是一种对CD4+炎性T细胞具有选择性化学吸引的趋化因子。本研究探讨了IL-16在克罗恩病和溃疡性结肠炎中的细胞表达。方法：应用逆转录聚合酶链反应、非放射性原位杂交和免疫组织化学分析克罗恩病、溃疡性结肠炎和正常对照组织样本中IL-16的表达。采用双染色法对表达IL-16的细胞进行表征。免疫组化法测定CD4+细胞的浸润量，逐级切片与相应的IL-16+细胞数相关。结果：与溃疡性结肠炎和对照探针相比，克罗恩病中IL-16+细胞数量增加。CD4和CD8阳性T细胞表达IL-16。此外，活动性克罗恩病中有大量的IL-16阳性肥大细胞。克罗恩病患者CD4+淋巴细胞数量的增加与IL- 16阳性细胞数量的增加呈正相关。结论：活动期克罗恩病患者T细胞和肥大细胞中IL-16表达升高与CD4+淋巴细胞数量增加有关。IL-16的局部表达似乎在克罗恩病炎症过程的开始和持续中起着重要作用，可能是通过IL-16介导的CD4+细胞（主要是淋巴细胞）进入肠壁的募集。
Background-The mechanisms involved in the initiation and maintenance of Crohn's disease are poorly understood. Previous studies have demonstrated an increased number of infiltrating CD4+ T cells within the inflammatory affected bowel wall in Crohn's disease. Novel therapy approaches using anti-CD4 antibodies are thought to be effective in Crohn's disease.Aims-Interleukin 16 (IL-16) has been characterised as a chemokine with selective chemoattraction for CD4+ inflammatory T cells. In this study, cellular expression of IL-16 in Crohn's disease and ulcerative colitis was investigated.Methods-Expression of IL-16 was analysed in tissue samples of Crohn's disease, ulcerative colitis, and normal controls by applying reverse transcription-polymerase chain reaction, non-radioactive in situ hybridisation, and immunohistochemistry. Double staining methods were used to characterise cells expressing IL-16. The amount of infiltrating CD4+ cells was determined by immunohistochemistry and correlated with the corresponding IL-16+ cell number by step sections.Results-An increased number of IL-16+ cells in Crohn's disease in comparison with ulcerative colitis and control probes was demonstrated. IL-16 was expressed by CD4 and CD8 positive T cells. In addition, in active Crohn's disease there was a substantial number of IL-16 positive mast cells. The increased number of CD4+ lymphocytes correlated positively with the increased number of IL- 16 positive cells in Crohn's disease.Conclusion-Our results demonstrate that increased expression of IL-16 in T cells and mast cells in active Crohn's disease is associated with increased numbers of CD4+ lymphocytes. Local expression of IL-16 seems to play a significant role in the initiation and persistence of the inflammatory process in Crohn's disease, presumably by IL-16 mediated recruitment of CD4+ cells, mostly lymphocytes, into the bowel wall.