Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique
Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique
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DOI：
10.1016/j.rbmo.2010.10.015
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发表时间：
2011-02-01
影响因子：
4
通讯作者：
Andersen, Claus Yding
中科院分区：
文献类型：
--
作者：
Rosendahl, Mikkel;Schmidt, Kirsten Tryde;Andersen, Claus Yding
本文介绍了丹麦10年（1999-2009）冷冻保存（n = 386）和卵巢组织自体移植（n = 18）的经验。在将该技术应用于人类之前，该方法经过了彻底的测试和验证。用四种不同的冷冻保护剂组合对小鼠和人卵巢组织进行冷冻后的组织学评价，选择冷冻保护剂溶液。通过将冷冻解冻的人卵巢组织（n = 49）移植到卵巢切除的裸鼠体内，证实了其生存能力。冷冻前4-5小时运输新鲜组织后的生存能力已经得到验证。冷冻保存前隔夜运输新鲜卵巢组织的评估，将人卵巢组织在冰上保存20小时，然后冷冻保存。将解冻后的卵巢组织移植到切除卵巢的裸鼠体内，组织学证实其存活能力。在丹麦，共有12名妇女接受了18例卵巢组织自体移植。所有妇女的卵巢功能都恢复了，两名妇女生下了三个健康的婴儿。在这两名妇女中，组织在冷冻保存前在冰上运输4-5小时。卵巢组织冷冻保存是保存生育能力的重要方法；然而，在临床应用该方法之前，每个实验室都应该对其技术进行彻底的验证。(C) 2010年，生殖保健有限公司。Elsevier Ltd.出版。版权所有。
This paper presents the Danish 10-year experience (1999-2009) with cryopreservation (n = 386) and autotransplantation of ovarian tissue (n = 18). Before applying the technique to humans, the method was thoroughly tested and validated. The cryoprotectant solution was chosen after histological evaluation of mouse and human ovarian tissue after freezing with four different combinations of cryoprotectants. Viability was confirmed by transplantation of frozen-thawed human ovarian tissue (n = 49) to oophorectomized Nude mice. Viability after transport of fresh tissue 4-5 h prior to freezing had previously been validated. Overnight transport of fresh ovarian tissue prior to cryopreservation was evaluated when human ovarian tissue was kept on ice for 20 h and then cryopreserved. The thawed ovarian tissue was transplanted to an oophorectomized Nude mouse and histology confirmed viability. In Denmark 12 women have received a total of 18 autotransplantations of ovarian tissue. All women resumed ovarian function and three healthy babies were born to two women. In both women, the tissue was transported on ice for 4-5 h prior to cryopreservation. Ovarian tissue cryopreservation is an important method for fertility preservation; however, before applying the method clinically, each laboratory should perform thorough validation of their technique. (C) 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.