A modular method for directing protein self-assembly.
A modular method for directing protein self-assembly.
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DOI：
10.1021/acssynbio.9b00504
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发表时间：
2020-04
影响因子：
4.7
通讯作者：
James A. J. Arpino;K. Polizzi
中科院分区：
文献类型：
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作者：
James A. J. Arpino;K. Polizzi
蛋白质是具有不同结构、电荷和功能的多功能大分子。它们是用于药物递送、生物传感或组织工程应用的生物材料的理想构建块。与此同时，开发化学工艺的绿色替代品的需求导致了对多酶生物催化路线的新兴趣，以获得精细、特殊和商品化学品。因此，使用蛋白质-蛋白质相互作用可靠地组装蛋白质复合物的方法将促进新材料的快速生产。在这里，我们展示了一种方法，用于模块化组装的蛋白质材料使用超电荷的蛋白质作为脚手架的“枢纽”，其上的目标蛋白质轴承带相反电荷的结构域已自组装。材料的物理特性可以通过混合、加热和分解来调整，这些都是通过pH值或盐浓度的变化来触发的。该系统可以扩展到生物材料的合成。我们的模块化方法可以用于可靠地指导蛋白质的自组装，使用小的带电标签结构域，可以很容易地编码在融合蛋白中。
Proteins are versatile macromolecules with diverse structure, charge, and function. They are ideal building blocks for biomaterials for drug delivery, biosensing, or tissue engineering applications. Simultaneously, the need to develop green alternatives to chemical processes has led to renewed interest in multi-enzyme biocatalytic routes to fine, specialty and commodity chemicals. Therefore, a method to reliably assemble protein complexes using protein-protein interactions would facilitate the rapid production of new materials. Here we show a method for modular assembly of protein materials using a super-charged protein as a scaffolding 'hub' onto which target proteins bearing oppositely charged domains have been self-assembled. The physical properties of the material can be tuned through blending and heating and disassembly triggered using changes in pH or salt concentration. The system can be extended to the synthesis of living materials. Our modular method can be used to reliably direct the self-assembly of proteins using small charged tag domains that can be easily encoded in a fusion protein.