Crystallization and preliminary X-ray diffraction analysis of the cytosolic domain of a cation diffusion facilitator family protein
Crystallization and preliminary X-ray diffraction analysis of the cytosolic domain of a cation diffusion facilitator family protein
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DOI：
10.1107/s1744309107038948
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发表时间：
2007-09-01
影响因子：
0.9
通讯作者：
Nureki, Osamu
中科院分区：
文献类型：
--
作者：
Hattori, Motoyuki;Tanaka, Yoshiki;Nureki, Osamu
阳离子扩散促进剂（CDF）家族蛋白广泛分布于生命和运输金属如锌和各种重金属的三个领域。Proximine CDF蛋白由N-末端推定的六跨膜结构域和随后的C-末端胞质结构域组成。海栖热袍菌CDF家族蛋白质的胞质结构域已经过表达、纯化和结晶。硒代甲硫氨酸取代的晶体使用同步辐射将X射线衍射至2.5埃分辨率，属于空间群R32，晶胞参数a = B = 97.7，c = 83.4埃，并且预期在每个不对称单元中包含一个分子。
The cation diffusion facilitator ( CDF) family proteins are ubiquitously distributed in the three domains of life and transport metals such as zinc and various heavy metals. Prokaryotic CDF proteins consists of an N-terminal putative six-transmembrane domain followed by a C-terminal cytosolic domain. The cytosolic domain of the CDF-family protein from Thermotoga maritima has been overexpressed, purified and crystallized. The selenomethionine-substituted crystals diffracted X-rays to 2.5 angstrom resolution using synchrotron radiation, belonged to space group R32, with unit-cell parameters a = b = 97.7, c = 83.4 angstrom, and are expected to contain one molecule in each asymmetric unit.