DNA synthesis on discontinuous templates by human DNA polymerases: implications for non-homologous DNA recombination.
DNA synthesis on discontinuous templates by human DNA polymerases: implications for non-homologous DNA recombination.
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人类 DNA 聚合酶在不连续模板上合成 DNA：对非同源 DNA 重组的影响。
DOI：
10.1093/nar/26.16.3729
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发表时间：
1998
影响因子：
14.9
通讯作者：
Morgan,WF
中科院分区：
文献类型：
--
作者：
Islas,L;Fairley,CF;Morgan,WF
DNA polymerases catalyze the synthesis of DNA using a continuous uninterrupted template strand. However, it has been shown that a 3′→5′ exonuclease-deficient form of the Klenow fragment ofEscherichia coliDNA polymerase I as well as DNA polymerase ofThermus aquaticuscan synthesize DNA across two unlinked DNA templates. In this study, we used an oligonucleotide-based assay to show that discontinuous DNA synthesis was present in HeLa cell extracts. DNA synthesis inhibitor studies as well as fractionation of the extracts revealed that most of the discontinuous DNA synthesis was attributable to DNA polymerase α. Additionally, discontinuous DNA synthesis could be eliminated by incubation with an antibody that specifically neutralized DNA polymerase α activity. To test the relative efficiency of each nuclear DNA polymerase for discontinuous synthesis, equal amounts (as measured by DNA polymerase activity) of DNA polymerases α, β, δ (± PCNA) and ε (±PCNA) were used in the discontinuous DNA synthesis assay. DNA polymerase α showed the most discontinuous DNA synthesis activity, although small but detectable levels were seen for DNA polymerases δ (+PCNA) and δ (− PCNA). Klenow fragment and DNA polymerase β showed no discontinuous DNA synthesis, although at much higher amounts of each enzyme, discontinuous synthesis was seen for both. Discontinuous DNA synthesis by DNA polymerase α was seen with substrates containing 3 and 4 bp single-strand stretches of complementarity; however, little synthesis was seen with blunt substrates or with 1 bp stretches. The products formed from these experiments are structurally similar to that seenin vivofor non-homologous end joining in eukaryotic cells. These data suggest that DNA polymerase α may be able to rejoin double-strand breaksin vivoduring replication.
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DOI：
10.1002/jbm.820221109
发表时间：
1988
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
B. Dupuy;H. Gin;C. Baquey;D. Ducassou
通讯作者：
D. Ducassou
甲基丙烯酸羟烷基酯热塑性聚合物的制备及表征
影响因子：
3
作者：
W. Stevenson;R. Evangelista;R. L. Broughton;M. Sefton
通讯作者：
M. Sefton
影响因子：
3.7
作者：
Henrick J. Malik;F. E. Croxton;D. Cowden;S. Klein
通讯作者：
Henrick J. Malik;F. E. Croxton;D. Cowden;S. Klein
微囊肿瘤测定：用于体内评估抗癌药物对人类肿瘤细胞系影响的新短期测定。
影响因子：
11.2
作者：
Gorelik,E;Ovejera,A;Shoemaker,R;Jarvis,A;Alley,M;Duff,R;Mayo,J;Herberman,R;Boyd,M
通讯作者：
Boyd,M
DOI：
10.1002/jbm.820240908
发表时间：
1990-09-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
CROOKS, CA;DOUGLAS, JA;SEFTON, MV
通讯作者：
SEFTON, MV