Analysis of individual human trigeminal ganglia for latent herpes simplex virus type 1 and varicella-zoster virus nucleic acids using real-time PCR
Analysis of individual human trigeminal ganglia for latent herpes simplex virus type 1 and varicella-zoster virus nucleic acids using real-time PCR
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DOI：
10.1128/jvi.74.24.11464-11471.2000
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发表时间：
2000-12-01
影响因子：
5.4
通讯作者：
Tal-Singer, R
中科院分区：
文献类型：
--
作者：
Cohrs, RJ;Randall, J;Tal-Singer, R
单纯疱疹病毒1型(HSV-1)和水痘-带状疱疹病毒(VZV)在初次感染后建立在周围神经系统的潜伏感染。在潜伏期间，两种病毒基因组都表现出有限的转录，在潜伏感染的人类神经节中一致地检测到HSV-1 LATs和至少四个VZV转录本。在这项研究中，我们使用实时荧光定量聚合酶链式反应检测了17例受试者三叉神经节(TG)中的病毒DNA拷贝数。同一个体左右Tg之间的HSV-1基因组数目没有显著差异，每个受试者的拷贝数从42.9到677.9拷贝/100ngDNA不等。同一个体左右Tg之间的VZV基因组数目也没有显著差异，每个受试者的拷贝数从37.0到3560.5拷贝/100 ng DNA。在含有潜伏HSV-1的神经节中持续检测到HSV-1 LAT转录本，并且相对表达差异达500倍。在分析的三个VZV转录本中，只有映射到基因63的转录本在潜伏感染的神经节中被持续检测到，并且相对表达差异是>；2000倍。因此，与HSV-1潜伏感染神经节中的LAT转录类似，VZV基因63的转录似乎是VZV潜伏的标志。
Herpes simplex virus type 1 (HSV-1) and varicella-zoster virus (VZV) establish latent infections in the peripheral nervous system following primary infection. During latency both virus genomes exhibit limited transcription, with the HSV-1 LATs and at least four VZV transcripts consistently detected in latently infected human ganglia. In this study we used real-time PCR quantitation to determine the viral DNA copy number in individual trigeminal ganglia (TG) from 17 subjects. The number of HSV-1 genomes was not significantly different between the left and right TG from the same individual and varied per subject from 42.9 to 677.9 copies per 100 ng of DNA. The number of VZV genomes was also not significantly different between left and right TG from the same individual and varied per subject from 37.0 to 3,560.5 copies per 100 ng of DNA. HSV-1 LAT transcripts were consistently detected in ganglia containing latent HSV-1 and varied in relative expression by > 500-fold. Of the three VZV transcripts analyzed, only transcripts mapping to gene 63 were consistently detected in latently infected ganglia and varied in relative expression by >2,000-fold. Thus, it appears that, similar to LAT transcription in HSV-1 latently infected ganglia, VZV gene 63 transcription is a hallmark of VZV latency.