High-Throughput Microchannels for Single Cell Immobilization
High-Throughput Microchannels for Single Cell Immobilization
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DOI：
10.1109/icarcv.2018.8581306
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发表时间：
2018-11
期刊：
影响因子：
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通讯作者：
Xiaoqing Tang;Xiaoming Liu;Pengyun Li;Yuqing Lin;Qiang Huang;T. Arai
中科院分区：
文献类型：
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作者：
Xiaoqing Tang;Xiaoming Liu;Pengyun Li;Yuqing Lin;Qiang Huang;T. Arai
目前，单细胞分析已成为获取细胞个体信息，研究细胞基因、蛋白质表达和代谢水平的随机性所引起的异质性的重要手段。许多单细胞分析的设备和技术已经被开发以满足这些需求。在本文中，我们提出了一个高通量的微通道，用于单细胞固定化与小的切压。它具有高密度阵列，可容纳多达130 × 300个陷阱在1 × 2平方毫米。实验结果表明，在优化的微通道结构下，单细胞在40 s内可以占据91%的捕获单元。因此，我们期望高通量微通道在生物学研究中具有重要意义。
Nowadays single cell analysis becomes a more and more important method to gather the information of individual cells and study the heterogeneity of cells caused by random expression of gene, protein and the level of metabolism. Many device and technologies of single cell analysis have been developed to meet these needs. In this paper, we presented a high-throughput microchannel for single cell immobilization with small sheer pressure. It features high density arrays, which can accommodate up to 130∼300 traps within 1∼2 mm2. According to our experiment, about 91% of capture unit can be occupied by the single cell in 40 s, using the optimized structure of microchannels. Therefore, we expect that the high throughput microchannels can be of great importance for the biological research.