Micromechanical detection of proteins using aptamer-based receptor molecules
Micromechanical detection of proteins using aptamer-based receptor molecules
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DOI：
10.1021/ac049859f
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发表时间：
2004-06-01
影响因子：
7.4
通讯作者：
Manalis, SR
中科院分区：
文献类型：
--
作者：
Savran, CA;Knudsen, SM;Manalis, SR
我们报告了使用微型悬臂传感器进行无标记蛋白质检测，该传感器通过 DNA 适体功能化以充当受体分子。该传感器利用两个相邻的悬臂构成传感器/参考对，并允许直接检测两个悬臂之间的差异弯曲。一根悬臂使用为 Taq DNA 聚合酶选择的适体进行功能化，而另一根悬臂则用单链 DNA 封闭。我们发现，聚合酶-适体结合会引起表面应力的变化，从而导致悬臂弯曲的差异，其范围为 3 至 32 nm，具体取决于配体浓度。传感器表面的蛋白质识别是特异性的，并且具有与溶液中类似的浓度依赖性。
We report label-free protein detection using a microfabricated cantilever-based sensor that is functionalized with DNA aptamers to act as receptor molecules. The sensor utilizes two adjacent cantilevers that constitute a sensor/reference pair and allows direct detection of the differential bending between the two cantilevers. One cantilever is functionalized with aptamers selected for Taq DNA polymerase while the other is blocked with single-stranded DNA. We have found that the polymerase-aptamer binding induces a change in surface stress, which causes a differential cantilever bending that ranges from 3 to 32 nm depending on the ligand concentration. Protein recognition on the sensor surface is specific and has a concentration dependence that is similar to that in solution.