Quince (Cydonia oblonga Miller) peel polyphenols modulate LPS-induced inflammation in human THP-1-derived macrophages through NF-κB, p38MAPK and Akt inhibition
Quince (Cydonia oblonga Miller) peel polyphenols modulate LPS-induced inflammation in human THP-1-derived macrophages through NF-κB, p38MAPK and Akt inhibition
复制标题
DOI：
10.1016/j.bbrc.2012.01.003
复制
发表时间：
2012-02-03
影响因子：
3.1
通讯作者：
Essafi, Makram
中科院分区：
文献类型：
--
作者：
Essafi-Benkhadir, Khadija;Refai, Amira;Essafi, Makram
慢性炎症是几种病理的标志，例如类风湿性关节炎、胃炎、炎性肠病、动脉粥样硬化和癌症。广泛的抗炎化学品已被用于治疗这些疾病，同时呈现高毒性和许多副作用。在这里，我们报告了一种无毒，具有成本效益的天然制剂，从突尼斯木瓜Cydonia oblonga米勒多酚提取物的抗炎作用。脂多糖（LPS）处理人THP-1衍生的巨噬细胞诱导高水平的促炎性细胞因子TNF-α和趋化因子IL-8的分泌，其被温柏皮多酚提取物以剂量依赖性方式抑制。同时，木瓜多酚增强了LPS处理的巨噬细胞分泌的抗炎细胞因子IL-10的水平。我们进一步证明，当木瓜多酚与LPS处理相关时，IL-6分泌的意外增加部分地导致多酚介导的TNF-α分泌抑制。生化分析表明，木瓜多酚提取物抑制LPS介导的三种主要细胞促炎效应物，核因子-κ B（NF-κ B），p38 MAPIC和Akt的激活。总的来说，我们的数据表明，木瓜皮多酚提取物诱导了有效的抗炎作用，这可能被证明对治疗炎性疾病有用，富含木瓜的方案可能有助于预防和改善此类疾病的治疗。(C)2012 Elsevier Inc. All rights reserved.
Chronic inflammation is a hallmark of several pathologies, such as rheumatoid arthritis, gastritis, inflammatory bowel disease, atherosclerosis and cancer. A wide range of anti-inflammatory chemicals have been used to treat such diseases while presenting high toxicity and numerous side effects. Here, we report the anti-inflammatory effect of a non-toxic, cost-effective natural agent, polyphenolic extract from the Tunisian quince Cydonia oblonga Miller. Lipopolysaccharide (LPS) treatment of human THP-1-derived macrophages induced the secretion of high levels of the pro-inflammatory cytokine TNF-alpha and the chemokine IL-8, which was inhibited by quince peel polyphenolic extract in a dose-dependent manner. Concomitantly, quince polyphenols enhanced the level of the anti-inflammatory cytokine IL-10 secreted by LPS-treated macrophages. We further demonstrated that the unexpected increase in IL-6 secretion that occurred when quince polyphenols were associated with LPS treatment was partially responsible for the polyphenols-mediated inhibition of TNF-alpha secretion. Biochemical analysis showed that quince polyphenols extract inhibited the LPS-mediated activation of three major cellular pro-inflammatory effectors, nuclear factor-kappa B (NF-kappa B), p38MAPIC and Akt. Overall, our data indicate that quince peel polyphenolic extract induces a potent anti-inflammatory effect that may prove useful for the treatment of inflammatory diseases and that a quince-rich regimen may help to prevent and improve the treatment of such diseases. (C) 2012 Elsevier Inc. All rights reserved.