CzcD is a heavy metal ion transporter involved in regulation of heavy metal resistance in Ralstonia sp strain CH34
CzcD is a heavy metal ion transporter involved in regulation of heavy metal resistance in Ralstonia sp strain CH34
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DOI：
10.1128/jb.181.22.6876-6881.1999
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发表时间：
1999-11-01
影响因子：
3.2
通讯作者：
Nies, DH
中科院分区：
文献类型：
--
作者：
Anton, A;Grosse, C;Nies, DH
Ralstonia sp.菌株CH 34的Czc系统通过CzcCB（2）A阳离子-质子反向转运蛋白催化的离子流出介导对钴、锌和镉的抗性。CzcD蛋白参与Czc系统的调节。它是一种具有至少四个跨膜α-螺旋的膜结合蛋白，并且是阳离子扩散促进剂（CDF）蛋白家族的一个亚家族的成员，其出现在所有三个生命结构域中。在罗尔斯通氏菌属中缺失czcD导致Czc系统的部分组成型表达，这是由于在诱导剂存在和不存在的情况下结构czcCBA基因的转录增加。CzcD缺失可以完全由CzcD和来自酿酒酵母的另外两种CDF蛋白ZRC 1 p和COT 1 p反式互补。所有这三种蛋白质介导的小，但显着的电阻，钴，锌，镉在罗尔斯顿氏菌，这种电阻是基于减少积累的阳离子。因此，CzcD出现抑制Czc系统的诱导阳离子的出口。
The Czc system of Ralstonia sp. strain CH34 mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB(2)A cation-proton antiporter. The CzcD protein is involved in the regulation of the Czc system. It is a membrane-bound protein with at least four transmembrane alpha-helices and is a member of a subfamily of the cation diffusion facilitator (CDF) protein family, which occurs in all three domains of life. The deletion of czcD in a Ralstonia sp. led to partially constitutive expression of the Czc system due to an increased transcription of the structural czcCBA genes, both in the absence and presence of inducers. The czcD deletion could be fully complemented in trans by CzcD and two other CDF proteins from Saccharomyces cerevisiae, ZRC1p and COT1p. All three proteins mediated a small but significant resistance to cobalt, zinc, and cadmium in Ralstonia, and this resistance was based on a reduced accumulation of the cations. Thus, CzcD appeared to repress the Czc system by an export of the inducing cations.