Tissue engineering of heart valves: In vitro experiences
Tissue engineering of heart valves: In vitro experiences
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DOI：
10.1016/s0003-4975(00)01255-8
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发表时间：
2000-07-01
影响因子：
4.6
通讯作者：
Mayer, JE
中科院分区：
文献类型：
--
作者：
Sodian, R;Hoerstrup, SP;Mayer, JE
背景组织工程是一种新方法，正在开发将自体细胞移植到可生物降解支架上的技术，最终在体外和体内形成新的功能组织。我们的实验室专注于心脏瓣膜的组织工程，我们已经用一种可生物降解的聚合物聚羟基脂肪酸酯制造了一种三叶心脏瓣膜支架。在本实验中，我们评估了这种支架材料的适用性以及体外调节以产生用于三叶心脏瓣膜的组织工程的活组织。我们从多孔聚羟基链烷酸酯（Meatabolix Inc，剑桥，MA）构建了生物可降解和生物相容的三叶心脏瓣膜支架。支架由圆柱形支架（1 x 15 x 20 mm内径）和瓣叶（0.3 mm厚）组成，通过热处理技术将瓣叶连接到支架上。用从绵羊颈动脉生长和扩增的血管细胞接种多孔心脏瓣膜支架（孔径100至240 μ m），并将其置于脉动流生物反应器中1、4和8天。对工程组织进行生化检查、环境扫描电镜和组织学分析。在脉动流生物反应器中，聚羟基脂肪酸酯可以同步开启和关闭，从而可以构建三叶心脏瓣膜支架。培养和脉动流暴露后，细胞生长到孔中并形成汇合层。细胞大多是活的，并在多孔心脏瓣膜支架的内部和外部之间形成结缔组织。此外，我们还通过羟脯氨酸测定和Movat染色的糖胺聚糖在体外脉动流条件下测定了细胞增殖（DNA测定）和生成胶原的能力。聚羟基链烷酸酯可用于制造多孔的、可生物降解的心脏瓣膜支架。细胞似乎是活的，细胞外基质的形成诱导后，脉动现在曝光。(Ann Thorac Surg 2000;70：140-4）（C）2000年，胸外科医师协会。
Background. Tissue engineering is a new approach, whereby techniques are being developed to transplant autologous cells onto biodegradable scaffolds to ultimately form new functional tissue in vitro and in vivo. Our laboratory has focused on the tissue engineering of heart valves, and we have fabricated a trileaflet heart valve scaffold from a biodegradable polymer, a polyhydroxyalkanoate. In this experiment we evaluated the suitability of this scaffold material as well as in vitro conditioning to create viable tissue for tissue engineering of a trileaflet heart valve.Methods. We constructed a biodegradable and biocompatible trileaflet heart valve scaffold from a porous polyhydroxyalkanoate (Meatabolix Inc, Cambridge, MA). The scaffold consisted of a cylindrical stent (1 x 15 x 20 mm inner diameter) and leaflets (0.3 mm thick), which were attached to the stent by thermal processing techniques. The porous heart valve scaffold (pore size 100 to 240 mu m) was seeded with vascular cells grown and expanded from an ovine carotid artery and placed into a pulsatile flow bioreactor for 1, 4, and 8 days. Analysis of the engineered tissue included biochemical examination, environmental scanning electron microscopy, and histology.Results. It was possible to create a trileaflet heart valve scaffold from polyhydroxyalkanoate, which opened and closed synchronously in a pulsatile flow bioreactor. The cells grew into the pores and formed a confluent layer after incubation and pulsatile flow exposure. The cells were mostly viable and formed connective tissue between the inside and the outside of the porous heart valve scaffold. Additionally, we demonstrated cell proliferation (DNA assay) and the capacity to generate collagen as measured by hydroxyproline assay and movat-stained glycosaminoglycans under in vitro pulsatile now conditions.Conclusions. Polyhydroxyalkanoates can be used to fabricate a porous, biodegradable heart valve scaffold. The cells appear to be viable and extracellular matrix formation was induced after pulsatile now exposure. (Ann Thorac Surg 2000;70:140-4) (C) 2000 by The Society of Thoracic Surgeons.