Role of phosphorylcholine in Streptococcus pneumoniae and nontypeable Haemophilus influenzae adherence to epithelial cells
Role of phosphorylcholine in Streptococcus pneumoniae and nontypeable Haemophilus influenzae adherence to epithelial cells
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DOI：
10.1016/j.anl.2018.11.003
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发表时间：
2019-08-01
影响因子：
1.7
通讯作者：
Kurono, Yuichi
中科院分区：
文献类型：
--
作者：
Iuchi, Hiroyuki;Ohori, Junichiro;Kurono, Yuichi
目的：磷胆碱(PC)是肺炎链球菌(Spn)和非分型流感嗜血杆菌(NTHi)的结构成分，与血小板活化因子受体(PAF-R)的黏附有关。此外，PC的高表达被认为与Spn和NTHi毒力有关。本研究检测PC表达对Spn和NTHi与上皮细胞黏附的影响，以明确针对PC的疫苗的潜在有效性。方法：应用27株Spn和22株NTHi过夜培养，用荧光激活细胞分选法检测PC的表达，将其分为两组：PC低表达组(PC-low)和PC高表达组(PC-High)。然后用底特律562细胞和BALB/c小鼠检测细菌粘附性。结果：经抗PC单抗(TEPC-15)、PAF-R拮抗剂(ABT-491)和PC锁孔帽状血蓝蛋白(PC-KLH)处理后，PC-High组Spn和NTHi的黏附力及NTHi的侵袭力均显著降低。结论：PC参与了Spn和NTHi的粘膜黏附，并参与了PC高菌株对NTHi的黏膜侵袭，而PC低菌株对鼻咽癌的侵袭作用不明显。这些结果表明，以PC为靶点的黏膜疫苗只影响PC高的Spn和NTHi菌株，而不干扰由非致病性PC低的细菌组成的上呼吸道共生细菌群。(C)2018爱思唯尔B.V.保留所有权利。
Objective: Phosphorylcholine (PC) is a structural component of Streptococcus pneumoniae (Spn) and nontypeable Haemophilus influenzae (NTHi), and is known to be associated with adherence through the platelet activating factor receptor (PAF-R). Furthermore, high PC expression is considered to be involved in Spn and NTHi virulence. In this study, we examined the influence of PC expression on the adherence of Spn and NTHi to epithelial cells in order to clarify the potential effectiveness of a vaccine targeting PC.Methods: Twenty-seven strains of Spn and twenty-two strains of NTHi were used, cultured overnight, and PC expression was evaluated by fluorescence activated cell sorting; the strains were divided into two groups: PC low expression (PC-low) and PC high expression (PC-high) groups. Bacterial adherence was then examined using Detroit 562 cells and BALB/c mice. Bacterial invasion was then examined in Detroit 562 cells.Results: The adherence of Spn and NTHi and invasion of NTHi in the PC-high group was significantly reduced by pretreatment with a monoclonal anti-PC antibody (TEPC-15), PAF-R antagonist (ABT-491), and PC-keyhole limpet hemocyanin (PC-KLH). However, such findings were not observed in the PC-low group.Conclusion: The present study suggests that PC is involved in the mucosal adhesion of Spn and NTHi, and the mucosal invasion of NTHi with PC-high strains, but not PC-low strains. These results suggest that a PC-targeting mucosal vaccine only affects PC-high Spn and NTHi strains and does not disturb commensal bacterial flora in the upper respiratory tract, which comprises nonpathogenic PC-low bacteria. (C) 2018 Elsevier B.V. All rights reserved.