Induced antitumor immunity against DMBA-4 metastatic mammary tumors in rats using laser immunotherapy
Induced antitumor immunity against DMBA-4 metastatic mammary tumors in rats using laser immunotherapy
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DOI：
10.1002/ijc.11501
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发表时间：
2003-12-20
影响因子：
6.4
通讯作者：
Nordquist, RE
中科院分区：
文献类型：
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作者：
Chen, WR;Jeong, SW;Nordquist, RE
诱导的抗肿瘤免疫是癌症，特别是转移性肿瘤的高效和长期治疗。激光免疫疗法被开发用于诱导这种免疫应答。它包括肿瘤内施用光吸收染料和特殊配制的免疫佐剂，然后用近红外激光进行非侵入性照射。用这种方法治疗大鼠中的DMBA-4转移性乳腺肿瘤导致原发性肿瘤的局部控制和未治疗的远处转移的根除。激光免疫治疗后，大鼠对肿瘤再攻击具有抵抗力，并产生免疫力，可过继转移。为了更好地理解在该肿瘤模型中诱导的免疫，使用冻融DMBA-4细胞裂解物进行免疫，然后在21天后进行肿瘤攻击。肿瘤细胞裂解物免疫延迟转移的出现，但不提供针对肿瘤攻击的免疫。在观察转移性肿瘤之前，还进行了原发性肿瘤的手术切除。原发性肿瘤的切除在改变肿瘤进展的过程中是不成功的。肿瘤在原发部位再次出现，并在多个偏远部位发生转移。相比之下，通过激光免疫疗法成功治疗的荷瘤大鼠经历了肿瘤消退和根除，并对相同类型的肿瘤细胞的反复攻击产生了强烈的抵抗力。我们的研究结果表明，激光免疫疗法可能通过诱导肿瘤特异性、持久的免疫来治疗转移性肿瘤。(C)2003 Wiley-Liss，Inc.
Induced antitumor immunity is a highly effective and long-term cure for cancer, particularly for metastatic tumors. Laser immunotherapy was developed to induce such an immunologic response. It involves intratumoral administration of a light-absorbing dye and a specially formulated immuno-adjuvant, followed by noninvasive irradiation of a near-infrared laser. Treatment of DMBA-4 metastatic mammary tumors in rats with this approach has resulted in local control of primary tumors and eradication of untreated distant metastases. After laser immunotherapy, rats were resistant to tumor rechallenge and developed immunity, which could be adoptively transferred. To better understand the immunity induced in this tumor model, immunization using freeze-thaw DMBA-4 cell lysates was performed, followed by tumor challenge 21 days later. Tumor cell lysate immunization delayed the emergence of metastases but did not provide immunity against the tumor challenge. Also performed was surgical resection of primary tumors before the observation of metastatic tumors. Removal of primary tumors was unsuccessful at changing the course of tumor progression. Tumors re-emerged at the primary sites, and metastases developed at multiple remote sites. In contrast, tumor-bearing rats successfully treated by laser immunotherapy experienced tumor regression and eradication and developed strong resistance to repeated challenges by tumor cells of the same type. Our results show that laser immunotherapy could have potential for the treatment of metastatic tumors by inducing tumor-specific, long-lasting immunity. (C) 2003 Wiley-Liss, Inc.