Studies on diuretic and hypouricemic effects of Orthosiphon stamineus methanol extracts in rats
Studies on diuretic and hypouricemic effects of Orthosiphon stamineus methanol extracts in rats
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DOI：
10.1016/j.jep.2008.04.015
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发表时间：
2008-08-13
影响因子：
5.4
通讯作者：
Asmawi, M. Z.
中科院分区：
文献类型：
--
作者：
Arafat, O. M.;Tham, S. Y.;Asmawi, M. Z.
研究目的：丹参Orthosiphon staineus(唇形科)是一种传统的民间药物，在东南亚被广泛用于治疗几种肾脏疾病，痛风和利尿剂。材料与方法：以SD大鼠为实验动物，观察不同甲醇提取物对不同剂量组大鼠的利尿作用。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。口服剂量的甲醇和甲醇：水(1：1)提取物。急性试验以氢氯噻嗪(10 mg/kg)为阳性对照。甲醇和甲醇水(1：1)提取物0.5g/kg灌胃，连续7d。测定不同时间段的累积尿量和电解质(Na+、K+)浓度。以不同剂量(0.25、0.5、1和2g/kg)口服甲醇：水提取物(1：1)，观察其对小鼠的降尿作用。以别嘌醇为阳性对照。结果：单次给药(2g/kg)后8h，血尿酸排泄量显著增加(P<0.05和P<0.01)，与利尿剂氢氯噻嗪相似。同时。重复灌胃0.5g/kg甲醇：水(1：1)提取物可显著增加尿量(第3天至第7天)(p<；0.01)和电解质排泄量(第2天至第7天)(p<；0.05和p<；0.01)。0.5g/kg、1g/kg和2g/kg的甲醇：水(1：1)提取液和标准别嘌醇均能降低高尿酸血症大鼠6h的血尿酸水平。结论：雄黄甲醇：水(1：1)提取液对大鼠有明显的利尿和降尿作用。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利。
Aim of the study: Orthosiphon stamineus (Labiatae) is a traditional folk medicine widely used in Southeast Asia for the treatment of several kidney disorders, gout and as a diuretic. This study was conducted to examine the diuretic and hypouricemic effects of orthosiphon stamineus leaf extracts.Materials and methods: The diuretic effect of different methanol extracts was examined by treating different groups of Sprague-Dawley rats with single (2 mic effects in rats. (c) 2008 Elsevier Ireland Ltd. All rights reserved.g/kg) oral doses of methanol and methanol:water (1: 1) extracts. Hydrochlorothiazide (10 mg/kg) was used as positive control in acute study. Methanol and methanol water (1: 1) extracts at 0.5 g/kg were administered for a period of 7 consecutive days. Cumulative urine volume and electrolytes (Na+ and K+) concentrations at different time intervals were measured. On the other hand, hypouricemic activity of methanol:water extract (1:1) was experimented using different oral single doses (0.25, 0.5, 1 and 2 g/kg). Allopurinol was used as positive control. Uric acid concentration in serum was analyzed by using RP-H PLC at 280 nm.Results: Sodium and potassium excretion increased significantly (p < 0.05 and < 0.01) in the first 8 h of treatment with a single dose (2g/kg) of the extracts in a pattern comparable to that of the known diuretic hydrochlorothiazide. Meanwhile. repeated administration of 0.5g/kg methanol:water (1:1) extract showed a significant increase in urine volume (from day 3 to day 7) (p < 0.01) and electrolytes excretion (Na+ and K+) from day 2 to day 7 (p < 0.05 and < 0.01). On the other hand, 0.5, 1 and 2g/kg of methanol:water (1:1) extract and the standard allopurinol reduced the serum urate level in hyperuricemic rats at hour 6.Conclusion: These results provided an evidence of the high tendency of methanol:water (1: 1) extract of Orthosiphon stamineus towards diuretic and hypouricemic effects in rats. (c) 2008 Elsevier Ireland Ltd. All rights reserved.