DEFINED RECOMBINANTS OF POLIOVIRUS AND COXSACKIE-VIRUS - SEQUENCE-SPECIFIC DELETIONS AND FUNCTIONAL SUBSTITUTIONS IN THE 5'-NONCODING REGIONS OF VIRAL RNAS
DEFINED RECOMBINANTS OF POLIOVIRUS AND COXSACKIE-VIRUS - SEQUENCE-SPECIFIC DELETIONS AND FUNCTIONAL SUBSTITUTIONS IN THE 5'-NONCODING REGIONS OF VIRAL RNAS
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DOI：
10.1016/0042-6822(88)90393-5
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发表时间：
1988-01-01
期刊：
影响因子：
3.7
通讯作者：
SEMLER, BL
中科院分区：
文献类型：
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作者：
JOHNSON, VH;SEMLER, BL
我们描述了一种脊髓灰质炎-柯萨奇重组病毒（PCV 110）的变异体的分离，该病毒含有一个带有嵌合5′-非编码区的基因组RNA。变异病毒[命名为PCV 110（1）]的生长和生物合成特性与重组病毒的原始温度敏感分离株[命名为PCV 110（4）]完全不同。对PCV 110（1）RNA的5′-非编码区进行核苷酸测序，发现嵌合基因组的替代柯萨奇病毒区域内存在4个碱基缺失，这可能导致该变异重组病毒丧失温度敏感性。此外，我们已经产生了新的重组病毒，其含有（1）脊髓灰质炎病毒基因组N66-N627区域内的柯萨奇病毒序列和（2）脊髓灰质炎病毒基因组中N1-N627的柯萨奇病毒序列。这些重组病毒在37° C下生长对温度不敏感，并且具有与野生型脊髓灰质炎病毒相似的生物合成特性。我们的研究结果提供了证据，肠道病毒基因组RNA的5′非编码区编码的复制酶识别信号不是严格的序列特异性。
We describe the isolation of a variant of a polio-coxsackie recombinant virus (PCV110) containing a genomic RNA with a chimeric 5′-noncoding region. The variant virus [designated PCV110(1)] has growth and biosynthetic properties that are quite different from the original, temperature-sensitive isolate of the recombinant virus [designated PCV110(4)]. Nucleotide sequencing of the 5′-noncoding region of RNA from PCV110(1) revealed a 4-base deletion within the substituted coxsackievirus region of the chimeric genome that may contribute to the loss of temperature sensitivity of this variant recombinant virus. In addition, we have generated new recombinant viruses that contain (1) coxsackievirus sequences within the N66–N627 region of the poliovirus genome and (2) coxsackievirus sequences substituted from N1–N627 in the poliovirus genome. These recombinant viruses are not temperature sensitive for growth at 37° and have biosynthetic properties similar to those of wild-type poliovirus. Our results provide evidence that replicase recognition signals encoded in the 5′ noncoding regions of enterovirus genomic RNAs are not strictly sequence specific.