Differentiation in MALDI-TOF MS and FTIR spectra between two pathovars of Xanthomonas oryzae
Differentiation in MALDI-TOF MS and FTIR spectra between two pathovars of Xanthomonas oryzae
复制标题
米黄单胞菌两个致病变种之间 MALDI-TOF MS 和 FTIR 光谱的区分
DOI：
10.1016/j.saa.2014.06.056
复制
发表时间：
2014-12-10
影响因子：
4.4
通讯作者：
Sun, Guochang
中科院分区：
文献类型：
--
作者：
Ge, Mengyu;Li, Bin;Sun, Guochang
水稻白黄单胞菌（Xanthomonasmyelopv.）Myzicola（Xoc）菌株在表型和遗传上密切相关，这使得基于表型和基于DNA的方法难以区分这两种致病变型。本研究利用MALDI-TOF MS和FTIR光谱的差异，建立了一种快速、准确的方法。MALDI-TOF MS分析显示，9个和10个峰分别对Xoo和Xoc具有特异性，其可用作生物标志物以鉴定和区分两种密切相关的致病型。红外光谱分析表明，两种不同的光致变色剂在光谱带频率和各官能团的吸收强度上都存在显著差异。特别地，在3433、2867、1273、1065、983和951 cm（-1）处的6个峰是Xoo菌株特异性的，而在1572 cm（-1）处的一个峰是Xoc菌株特异性的。总的来说，本研究首次尝试识别和区分X的两种致病型。基于质谱和红外光谱的水稻细菌鉴定方法的建立，将有助于这两种病害的早期检测和防治。异源变种。(C)2014爱思唯尔有限公司版权所有。
Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas myzae pv. myzicola (Xoc) strains are closely related phenotypically and genetically, which make it difficult to differentiate between the two pathovars based on phenotypic and DNA-based methods. In this study, a fast and accurate method was developed based on the differences in MALDI-TOF MS and FTIR spectra between the two pathovars. MALDI-TOF MS analysis revealed that 9 and 10 peaks are specific to Xoo and Xoc, respectively, which can be used as biomarkers to identify and differentiate the two closely related pathovars. Furthermore, FTIR analysis showed that there is a significant difference in both the band frequencies and absorption intensity of various functional groups between the two pathovars. In particular, the 6 peaks at 3433, 2867, 1273, 1065, 983 and 951 cm(-1) were specific to the Xoo strains, while one peak at 1572 cm(-1) was specific to the Xoc strains. Overall, this study gives the first attempt to identify and differentiate the two pathovars of X. myzae based on mass and FIR spectra, which will be helpful for the early detection and prevention of the two rice diseases caused by both X. oryzae pathovars. (C) 2014 Elsevier B.V. All rights reserved.