Recognition of chromosome 6-associated target structures by human lymphokine-activated killer cells.
Recognition of chromosome 6-associated target structures by human lymphokine-activated killer cells.
复制标题
人类淋巴因子激活的杀伤细胞识别 6 号染色体相关的靶结构。
DOI：
10.4049/jimmunol.147.6.2063
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发表时间：
1991
影响因子：
4.4
通讯作者：
J. Schröder
中科院分区：
文献类型：
--
作者：
T. Helander;T. Timonen;P. Kalliomäki;J. Schröder
用不同群体的未刺激和IL-2激活的PBL对含有不同人类染色体的体细胞杂交鼠/人淋巴细胞进行结合和杀伤实验。未受刺激的PBL效应细胞对杂交瘤细胞和小鼠亲本细胞的结合活性和杀伤活性均较低。然而，IL-2激活的杀伤细胞(LAK)与携带人6号染色体的杂交瘤细胞结合并有效地杀伤，但对小鼠亲代细胞和不携带人6号染色体的杂交瘤细胞均无效。这些结果表明，人LAK细胞而不是内源性NK细胞结合并杀伤含有人6号染色体的鼠/人淋巴细胞杂交瘤细胞。因此，我们认为LAK细胞识别6号染色体基因编码的配体。
Different populations of unstimulated and IL-2-activated PBL were used in binding and killing assays against somatic mouse/human lymphocyte cell hybrids containing different human chromosomes. Unstimulated PBL effector cells showed low binding and killing activity to both cell hybrids and mouse parental cell lines. However, IL-2-activated killer (LAK) cells bound strongly to, and effectively killed, cell hybrids carrying human chromosome 6, but were inefficient in both assays to mouse parental cells and to cell hybrids not carrying human chromosome 6. These results show that human LAK cells but not endogenous NK cells bind and kill mouse/human lymphocyte hybrids containing human chromosome 6. We thus suggest that LAK cells recognize ligands encoded by genes on chromosome 6.