A flash-annealing technique to improve diffraction limits and lower mosaicity in crystals of glycerol kinase.
A flash-annealing technique to improve diffraction limits and lower mosaicity in crystals of glycerol kinase.
复制标题
一种闪光退火技术，可改善甘油激酶晶体的衍射极限并降低镶嵌性。
DOI：
10.1107/s0907444998004697
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Hol,WG
中科院分区：
文献类型：
--
作者：
Yeh,JI;Hol,WG
提出了一种闪光退火法，提高了晶体的衍射极限，同时降低了甘油激酶晶体的马赛克。这项技术利用了晶体在冷氮气中的短暂解冻和快速冻结循环。有效分辨率极限几乎增加了0.8% ä，从3.6%提高到2.8%，马赛克数值减半。
A flash-annealing method has been developed that increases the diffraction limits, and simultaneously decreases the mosaicity of glycerol kinase crystals. This technique utilizes brief thawing and rapid freezing cycles of the crystal in the cold nitrogen stream. The effective resolution limits increased almost by 0.8 Å, from 3.6 to 2.8 Å, and mosaicity values halved.