A fluorescence temperature-jump study of conformational transitions in myosin subfragment 1.
A fluorescence temperature-jump study of conformational transitions in myosin subfragment 1.
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肌球蛋白亚片段 1 构象转变的荧光跳温研究。
DOI：
10.1042/bj3580165
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
J. Wray
中科院分区：
文献类型：
--
作者：
C. Urbanke;J. Wray
在快速温度跳跃后，测量了来自兔和鸡骨骼肌的未修饰的肌球蛋白亚片段1（S1）与各种核苷酸和磷酸类似物结合的色氨酸荧光。随着温度的升高，荧光强度降低。在某些条件下，这种减少之后是增加，反映了蛋白质内的结构转变。在腺苷5 '-[β，γ-亚氨基]三磷酸（p[NH]ppA）或ADP和BeF（x）结合的情况下，这种上升非常迅速（倒数时间常数约为1.5）。2000 s（-1）），并且仅随起始温度略有变化，这表明，使用这些配体，两种不同的蛋白质构象在大的温度范围内快速平衡。在ATP的存在下，瞬态包括几个松弛过程。总体而言，结果表明，复合物的S1与ATP或与一些其他配体存在的两种形式的混合物中的温度依赖性的平衡。这些结果揭示了在最近的晶体学研究中发现的不同形式的S1，并表明肌球蛋白的结构状态和核苷酸结合的性质之间惊人地缺乏强耦合。
The tryptophan fluorescence of unmodified myosin subfragment 1 (S1) from rabbit and chicken skeletal muscle with various nucleotides and phosphate analogues bound was measured after rapid temperature jumps. The fluorescence decreased during the temperature rise. Under some conditions, this decrease was followed by an increase, reflecting structural transitions within the protein. With adenosine 5'-[beta,gamma-imido]triphosphate (p[NH]ppA) or with ADP and BeF(x) bound, this rise was very rapid (reciprocal time constant approx. 2000 s(-1)) and varied only slightly with starting temperature, suggesting that, with these ligands, two different protein conformations were present in rapid equilibrium over a large temperature range. In the presence of ATP, the transient included several relaxation processes. Overall, the results suggest that complexes of S1 with ATP or with a number of other ligands exist as a mixture of two forms in temperature-dependent equilibrium. The results throw light on the finding of different forms of S1 in recent crystallographic studies and indicate a surprising lack of strong coupling between myosin's structural state and the nature of the nucleotide bound.
1-N6-乙烯腺苷核苷酸与骨骼肌和平滑肌的肌球蛋白亚片段 1 和肌动亚片段 1 的反应。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rosenfeld,SS;Taylor,EW
通讯作者：
Taylor,EW
影响因子：
2.9
作者：
Gulick, AM;Bauer, CB;Rayment, I
通讯作者：
Rayment, I
腺苷 5-二磷酸和腺苷 5-（β，γ-亚胺三磷酸）与亚片段 1 和 acto 亚片段 1 结合的瞬时动力学。
影响因子：
2.9
作者：
Trybus,KM;Taylor,EW
通讯作者：
Taylor,EW