Model substrates for an RNA enzyme.
Model substrates for an RNA enzyme.
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RNA 酶的模型底物。
DOI：
10.1126/science.2443980
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Altman,S
中科院分区：
文献类型：
--
作者：
McClain,WH;Guerrier-Takada,C;Altman,S
M1 RNA是大肠杆菌核酸酶P的催化RNA亚基，可以切割缺乏正常tRNA序列特定结构域的新型转移RNA （tRNA）前体。被有效切割的最小tRNA前体仅保留氨基酸受体茎和T茎和环的结构域。3 '端CCA核苷酸残基在新型和正常tRNA前体加工中的重要性表明，M1 RNA参与了相同的酶促功能。
M1 RNA, the catalytic RNA subunit ofEscherichia coliribonuclease P, can cleave novel transfer RNA (tRNA) precursors that lack specific domains of the normal tRNA sequence. The smallest tRNA precursor that was cleaved efficiently retained only the domain of the amino acid acceptor stem and the T stem and loop. The importance of the 3′ terminal CCA nucleotide residues in the processing of both novel and normal tRNA precursors implies that the same enzymatic function of M1 RNA is involved.