"Fluorescent timer": Protein that changes color with time
"Fluorescent timer": Protein that changes color with time
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DOI：
10.1126/science.290.5496.1585
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发表时间：
2000-11-24
期刊：
影响因子：
56.9
通讯作者：
Siebert, P
中科院分区：
文献类型：
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作者：
Terskikh, A;Fradkov, A;Siebert, P
我们生成了红色荧光蛋白 drFP583 的突变体。随着时间的推移，突变体 (E5) 的荧光从绿色变为红色。颜色转换率与蛋白质浓度无关，因此可用于追踪时间依赖性表达。我们使用 E5 体内标记来测量秀丽隐杆线虫中热休克依赖性启动子和发育中爪蟾胚胎中 Otx-2 启动子的表达。因此，E5是一种“荧光计时器”，可用于监测整个生物体范围内靶启动子的激活和下调。
We generated a mutant of the red fluorescent protein drFP583. The mutant (E5) changes its fluorescence from green to red over time. The rate of color conversion is independent of protein concentration and therefore can be used to trace time-dependent expression. We used in vivo labeling with E5 to measure expression from the heat shock-dependent promoter in Caenorhabditis elegans and from the Otx-2 promoter in developing Xenopus embryos. Thus, E5 is a "fluorescent timer" that can be used to monitor both activation and downregulation of target promoters on the whole-organism scale.