Polyoxometalate nanostructured gold surfaces for sensitive biosensing of benzo[a]pyrene
Polyoxometalate nanostructured gold surfaces for sensitive biosensing of benzo[a]pyrene
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DOI：
10.1016/j.snb.2014.12.015
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发表时间：
2015-03-31
影响因子：
8.4
通讯作者：
Boujday, Souhir
中科院分区：
文献类型：
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作者：
Mercier, Dimitri;Ben Haddada, Maroua;Boujday, Souhir
设计了一种用于苯并[a]芘（B[a]P）检测的聚氧乙烯酸盐纳米结构免疫传感器。将带有两个胺官能团的有机-无机杂化聚氧乙烯酸盐（POM）（NBu 4）（3）[PW 11 O-39 {（SiC 6 H4 NH 2）（2）O}]共价连接到功能化的金基底上，以在其表面实现胺基团的纳米组织。随后将芘丁酸（PBA）接枝到胺基上以产生传感层。使用单克隆抗B [a]P抗体进行间接竞争形式的B[a]P检测，其与免疫探针的结合用石英晶体微天平与耗散测量（QCM-D）进行监测。POM-纳米结构的生物传感器的性能进行了比较，从半胱胺自组装单层构造的参考传感器。QCM-D测量显示了来自POM纳米结构化的重要输入。类似物在表面上的可及性和分析性能都得到增强，显示出这种纳米结构化策略对小分子生物传感的有希望的效果。(C)2014爱思唯尔有限公司版权所有。
We report the design of a polyoxometalate-nanostructured immunosensor for benzo[a]pyrene (B[a]P) detection. The organic-inorganic hybrid polyoxometalate (POM) (NBu4)(3)[PW11 O-39 {(SiC6H4NH2)(2)O}] carrying two amine functions was covalently attached to a functionalized gold substrate to achieve a nanometric organization of amine groups at its surface. Pyrenebutyric acid (PBA) was subsequently grafted to amine groups to create the sensing layer. The detection of B[a]P in the indirect competitive format was carried out using a monoclonal anti-B[a]P antibody whose binding to the immunoprobe was monitored with a quartz crystal microbalance with dissipation measurement (QCM-D). The performances of the POM-nanostructured biosensor were compared to a reference sensor constructed from a cysteamine self-assembled monolayer. QCM-D measurements displayed significant input from POM-nanostructuration. Both the accessibility of the analogue on the surface and the analytical performances were enhanced showing a promising effect of this strategy of nanostructuration for the biosensing of small molecules. (C) 2014 Elsevier B.V. All rights reserved.