DNA recovery and direct detection of Tn5 sequences from soil
DNA recovery and direct detection of Tn5 sequences from soil
复制标题
土壤中 Tn5 序列的 DNA 回收和直接检测
DOI：
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发表时间：
1991
影响因子：
2.4
通讯作者：
W. Klingmüller
中科院分区：
文献类型：
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作者：
S. Selenska;W. Klingmüller
在接种肠杆菌70 d后，在土壤中直接回收的EcoRI消化DNA中检测到插入到凝聚肠杆菌基因组中的特定Tn5序列，此时这些DNA不再可在琼脂培养基上培养。采用了一种新的土壤DNA提取方法。不需要PCR扩增DNA序列。
Specific Tn5 sequences inserted in the genome of Enterobacter agglomerans were detected in EcoRI digested DNA directly recovered from soil 70 d after its inoculation with the bacteria, when these were no longer culturable on agar medium. A new method of DNA extraction from soil was used. No amplification of DNA sequences by PCR was needed.