Quantification of amphetamine-induced changes in [C-11]raclopride binding with continuous infusion
Quantification of amphetamine-induced changes in [C-11]raclopride binding with continuous infusion
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DOI：
10.1097/00004647-199704000-00009
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发表时间：
1997-04-01
影响因子：
6.3
通讯作者：
Eckelman, WC
中科院分区：
文献类型：
--
作者：
Carson, RE;Breier, A;Eckelman, WC
Positron emission tomography and single photon emission computer tomography receptor-binding ligands can be used to measure changes in neurotransmitter levels. In particular, amphetamine-induced dopamine release has been assessed with [C-11]raclopride by paired bolus injections and with [I-123]iodobenzamide by using a single bolus plus infusion (B/I) study. Here, we measured the change in [C-11]raclopride-specific binding in rhesus monkeys after i.v. administration of 0.4 mg/kg amphetamine by using both the bolus and B/I paradigms. Paired bolus studies (control and postamphetamine) were analyzed using compartment modeling and graphical analysis with a new plasma metabolite model to measure the total distribution volume (V-T). Specific binding, calculated with three measures linearly proportional to the binding potential, demonstrated a 22-42% reduction in the postamphetamine study. V-T values from B/I studies were determined by the tissue-to-plasma ratio at equilibrium, in addition to the bolus methods. There was good agreement between the control V-T values between bolus and B/I studies. The amphetamine-induced change in specific binding in B/I studies was 19 +/- 16%, measured directly from tissue radioactivity levels. This study demonstrates that stimulus-induced changes in specific binding can be measured with a single [C-11]raclopride study using the B/I method.
