The effect of n-3 long-chain polyunsaturated fatty acids and lipoic acid on the heart in the ovariectomized rat model of menopause
The effect of n-3 long-chain polyunsaturated fatty acids and lipoic acid on the heart in the ovariectomized rat model of menopause
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DOI：
10.1080/10715762.2019.1620938
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发表时间：
2019-05-31
影响因子：
3.3
通讯作者：
Hackenhaar, Fernanda S.
中科院分区：
文献类型：
--
作者：
Marinho, Priscila M.;Salomon, Tiago B.;Hackenhaar, Fernanda S.
绝经是卵巢衰老导致雌激素水平下降的结果。雌激素水平的降低与氧化还原系统的损害相结合，可能导致绝经后心血管疾病的风险增加。补充抗氧化剂可能是降低心血管风险的一种选择。该研究评估了膳食补充二十二碳六烯酸（DHA），二十碳五烯酸（EPA）和α-硫辛酸（LA）16周对卵巢切除3个月大大鼠心脏中氧化应激生物标志物的影响。卵巢切除术并没有增加损伤标志物丙二醛和羰基的水平，并且两者都被LA补充降低。卵巢切除术增加了内源性抗氧化剂谷胱甘肽，维生素C和H2 O2的消耗水平，恢复后的DHA，EPA和LA补充。维生素E、谷胱甘肽过氧化物酶、谷胱甘肽-S-转移酶和超氧化物歧化酶不受卵巢切除术的影响。卵巢切除术后脂质和蛋白质损伤没有增加，部分内源性抗氧化剂伴随增加，表明心脏可能受到这些抗氧化剂的保护。DHA，EPA和LA恢复了这些内源性抗氧化剂，表明所有评估的补充剂都能有效调节心脏中的抗氧化剂氧化还原系统。LA对氧化还原标记物、降低脂质和蛋白质损伤标记物具有额外的作用。
Menopause occurs as consequence of ovarian senescence that leads to a drop of oestrogen hormone. The decreased oestrogen levels combined with the impairment of the redox system may contribute to the increased risk of postmenopausal cardiovascular disease. Supplementation with antioxidants may be an alternative to reduce cardiovascular risk. The study evaluated the effect of dietary supplementation with docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and alpha-lipoic acid (LA) for a period of 16 weeks on oxidative stress biomarkers in the hearts of ovariectomized 3-month-old rats. Ovariectomy did not increase the level of the damage markers malondialdehyde and carbonyl, and both were decreased by LA supplementation. Ovariectomy increased the levels of the endogenous antioxidants glutathione, vitamin C and H2O2 consumption, after restoration by DHA, EPA, and LA supplementation. Vitamin E, glutathione peroxidase, glutathione-S-transferase, and superoxide dismutase are not altered by ovariectomy. Lipid and protein damage are not increased after ovariectomy and a portion of the endogenous antioxidants concomitantly increased, suggesting that hearts may be protected by these antioxidants. DHA, EPA, and LA restored these endogenous antioxidants, showing that all evaluated supplements are effective in modulating the antioxidant redox system in the heart. LA showed additional effect on redox markers, decreasing lipid and protein damage markers.