Bioactive glass nanofiber-collagen nanocomposite as a novel bone regeneration matrix
Bioactive glass nanofiber-collagen nanocomposite as a novel bone regeneration matrix
复制标题
DOI：
10.1002/jbm.a.30848
复制
发表时间：
2006-12-01
影响因子：
4.9
通讯作者：
Kim, Hyoun-Ee
中科院分区：
文献类型：
--
作者：
Kim, Hae-Won;Song, Ju-Ha;Kim, Hyoun-Ee
纳米级有机-无机纳米复合系统在开发具有先进性能的生物材料方面具有巨大潜力。在此，我们开发了一种新型纳米复合生物材料，由生物活性玻璃纳米纤维（BGNF）和胶原重构纤维基质组成，用于骨再生医学。将具有生物活性成分（58SiO(2)中心点38CaO中心点4P(2)O(5)）的溶胶-凝胶衍生玻璃电纺成平均直径接近320 nm的纳米级纤维。随后 BGNF 与 I 型胶原蛋白杂交，I 型胶原蛋白是骨基质的主要有机成分。将 BGNF 和自组装胶原溶胶在水溶液中混合，然后通过采用适当的加工条件交联产生薄膜或大孔支架形式的 BGNF-胶原纳米复合材料。观察到 BGNF 均匀分布在胶原重建的纳米纤维基质内。当在模拟体液中孵育时，纳米复合材料基质诱导其表面快速形成骨状磷灰石矿物质，在体外表现出优异的生物活性。成骨细胞在 BGNF-胶原纳米复合材料上显示出良好的生长。特别是，纳米复合材料上细胞的碱性磷酸酶活性明显高于胶原蛋白上的细胞碱性磷酸酶活性。这种新型 BGNF-胶原纳米复合材料被认为在骨再生和组织工程应用中具有巨大潜力。 (c) 2006 年 Wiley 期刊公司
Nanoscale organized organic-inorganic nanocomposite systems have great potential in the development of biomaterials with advanced properties. Herein, we developed a novel nanocomposite biomaterial consisting of bioactive glass nanofiber (BGNF) and collagen reconstituted fibrous matrix for bone regenerative medicine. A sol-gel derived glass with a bioactive composition (58SiO(2)center dot 38CaO center dot 4P(2)O(5)) was electrospun to a nanoscale fiber with an average diameter of similar to 320 nm. The BGNF was subsequently hybridized with type I collagen, which is the main organic constituent of bone matrix. The BGNF and self-assembled collagen sol were combined in aqueous solution, and then crosslinked to produce a BGNF-collagen nanocomposite, in the form of either a thin membrane or a macroporous scaffold, by adopting appropriate processing conditions. The BGNF was observed to be distributed uniformly within the collagen reconstituted nanofibrous matrix. The nanocomposite matrices induced rapid formation of bone-like apatite minerals on their surfaces when incubated in a simulated body fluid, exhibiting excellent bioactivity in vitro. Osteoblastic cells showed favorable growth on the BGNF-collagen nanocomposite. In particular, the alkaline phosphatase activity of the cells on the nanocomposite was significantly higher than that on the collagen. This novel BGNF-collagen nanocomposite is believed to have significant potential in bone regeneration and tissue engineering applications. (c) 2006 Wiley Periodicals, Inc.