Species-Wide Variation in Shoot Nitrate Concentration, and Genetic Loci Controlling Nitrate, Phosphorus and Potassium Accumulation in Brassica napus L.
Species-Wide Variation in Shoot Nitrate Concentration, and Genetic Loci Controlling Nitrate, Phosphorus and Potassium Accumulation in Brassica napus L.
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DOI：
10.3389/fpls.2018.01487
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发表时间：
2018
影响因子：
5.6
通讯作者：
Graham NS
中科院分区：
文献类型：
--
作者：
Alcock TD;Havlickova L;He Z;Wilson L;Bancroft I;White PJ;Broadley MR;Graham NS
许多作物系统都使用大量的氮、磷和钾肥。确定控制养分积累的遗传位点可能有助于作物育种策略，以提高肥料利用效率，降低财务和环境成本。在这里，叶片硝酸盐浓度的变化在383个基因型的甘蓝型油菜的多样性人口的特点。控制叶片硝酸盐，磷和钾浓度变化的遗传位点，然后通过关联转录组学使用单核苷酸多态性（SNP）标记和基因表达标记（GEMs）确定。叶片硝酸盐浓度变化超过8倍，在整个多样性人口。经过FDR校正后，共发现455个SNP标记与叶片硝酸盐浓度相关。在连锁不平衡的高度相关的标记是一些已知的硝酸盐转运蛋白和传感器，包括一个基因被认为介导的主要硝酸盐转运蛋白NRT1.1的表达。影响根和根毛发育的几个基因与叶磷浓度相关的染色体区域共定位。三个ABC-转运蛋白的直系同源物参与木栓质合成的根也共定位与叶硝酸盐和磷的协会峰。在附近的等位基因变异，高度相关的SNPs赋予叶片硝酸盐和磷浓度的变化很大。总共有五个GEM与叶钾浓度FDR校正后，包括GEM，对应于生长素反应家族蛋白。本研究所确定的候选基因座、基因和有利等位基因可用于标记辅助选择策略，以提高B的肥料利用效率。油菜。
Large nitrogen, phosphorus and potassium fertilizer inputs are used in many crop systems. Identifying genetic loci controlling nutrient accumulation may be useful in crop breeding strategies to increase fertilizer use efficiency and reduce financial and environmental costs. Here, variation in leaf nitrate concentration across a diversity population of 383 genotypes of Brassica napus was characterized. Genetic loci controlling variation in leaf nitrate, phosphorus and potassium concentration were then identified through Associative Transcriptomics using single nucleotide polymorphism (SNP) markers and gene expression markers (GEMs). Leaf nitrate concentration varied over 8-fold across the diversity population. A total of 455 SNP markers were associated with leaf nitrate concentration after false-discovery-rate (FDR) correction. In linkage disequilibrium of highly associated markers are a number of known nitrate transporters and sensors, including a gene thought to mediate expression of the major nitrate transporter NRT1.1. Several genes influencing root and root-hair development co-localize with chromosomal regions associated with leaf P concentration. Orthologs of three ABC-transporters involved in suberin synthesis in roots also co-localize with association peaks for both leaf nitrate and phosphorus. Allelic variation at nearby, highly associated SNPs confers large variation in leaf nitrate and phosphorus concentration. A total of five GEMs associated with leaf K concentration after FDR correction including a GEM that corresponds to an auxin-response family protein. Candidate loci, genes and favorable alleles identified here may prove useful in marker-assisted selection strategies to improve fertilizer use efficiency in B. napus.
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影响因子：
11.6
作者：
Hamburger, D;Rezzonico, E;Poirier, Y
通讯作者：
Poirier, Y
影响因子：
5.8
作者：
Berry, P. M.;Spink, J.;White, P. J.
通讯作者：
White, P. J.
影响因子：
5.6
作者：
Bus A;Körber N;Parkin IA;Samans B;Snowdon RJ;Li J;Stich B
通讯作者：
Stich B
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
5.2
作者：
Daras, Gerasimos;Rigas, Stamatis;Hatzopoulos, Polydefkis
通讯作者：
Hatzopoulos, Polydefkis