Interaction of rotavirus with human peripheral blood mononuclear cells:: Plasmacytoid dendritic cells play a role in stimulating memory rotavirus specific T cells in vitro
Interaction of rotavirus with human peripheral blood mononuclear cells:: Plasmacytoid dendritic cells play a role in stimulating memory rotavirus specific T cells in vitro
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DOI：
10.1016/j.virol.2007.04.007
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发表时间：
2007-09-15
期刊：
影响因子：
3.7
通讯作者：
Angel, Juana
中科院分区：
文献类型：
--
作者：
Mesa, Martha C.;Rodriguez, Luz-Stella;Angel, Juana
我们研究了 RV 与成年志愿者的人外周血单核细胞 (PBMC) 的相互作用。将 PBNIC 暴露于恒河猴 RV (RRV) 后，T 和 B 淋巴细胞、NK 细胞、单核细胞以及骨髓和浆细胞样树突状细胞以不同水平表达 RV 非结构蛋白。如果在抗 VP7 中和抗体或 10% 自体血清存在的情况下进行感染，这些 RV 蛋白的表达就会消失。 RRV暴露的PBMC的上清液含有TNF-α、IL-6、IFN-α、IFNγ、IL-γ和IL-10。发现浆细胞样 DC 是 IFN-α 产生的主要来源，在缺乏它们的情况下，IFN-γ 的产生以及分泌 IFN-γ 的 RV 特异性 T 细胞的频率减少。最后，我们无法检测到与急性 RV 感染儿童的 PBMC 相关的 RV 抗原或 PBMC 中 RV 非结构蛋白的表达。因此，尽管 PBMC 对 RV 感染的初始阶段很敏感，但大多数患有 RV 胃肠炎的儿童的 PBNIC 并未受到感染。 (c) 2007 Elsevier Inc. 保留所有权利。
We studied the interaction of RV with human peripheral blood mononuclear cells (PBMC) from adult volunteers. After exposure of PBNIC to rhesus RV (RRV), T and B lymphocytes, NK cells, monocytes, and myeloid and plasmacytoid dendritic cells expressed RV non-structural proteins, at variable levels. Expression of these RV proteins was abolished if infection was done in the presence of anti-VP7 neutralizing antibodies or 10% autologous serum. Supernatants of RRV exposed PBMC contained TNF-alpha, IL-6, IFN-alpha, IFN gamma, IL-gamma and IL-10. Plasmacytoid DC were found to be the main source of IFN-a production, and in their absence the production of IFN-gamma and the frequency of RV specific T cells that secrete IFN-gamma diminished. Finally, we could not detect RV-antigen associated with the PBMC or expression of RV non-structural proteins in PBMC of acutely RV-infected children. Thus, although PBMC are susceptible to the initial steps of RV infection, most PBNIC of children with RV-gastroenteritis are not infected. (c) 2007 Elsevier Inc. All rights reserved.