First Evidence of the Existence of Semi-Cryptic Species and of a Phylogeographic Structure in the Gomphonema parvulum (Kutzing) Kutzing Complex (Bacillariophyta)
First Evidence of the Existence of Semi-Cryptic Species and of a Phylogeographic Structure in the Gomphonema parvulum (Kutzing) Kutzing Complex (Bacillariophyta)
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DOI：
10.1016/j.protis.2013.07.005
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发表时间：
2013-09-01
期刊：
影响因子：
2.5
通讯作者：
Humbert, Jean-Francois
中科院分区：
文献类型：
--
作者：
Kermarrec, Lenaig;Bouchez, Agnes;Humbert, Jean-Francois
小异极藻复合体包括具有相当大的形态变异性和广泛的地理分布的物种。这些特征使它们难以通过显微镜进行鉴定，并提出了它们的分类有效性和它们之间的地理差异的可能性的问题。在这种情况下，我们分离出39 G。parvulum s. l来自热带岛屿（马约特岛）和欧洲大陆河流的菌株。通过对ITS、rbcL和cox 1 3个片段的测序，可清楚地鉴定出4个进化枝（A、B、C和D），另外一个进化枝（B '）仅在rbcL序列上可区分。主要的四个分支可以通过它们的形态标准，特别是通过中心区域的形状来分离，但它们之间也有一些重叠。因此，我们认为G. parvulum复合体包含至少四个半隐种，对应于四个主要分支。其中一种（A）仅在马约特岛发现，而另外两种（C和D）仅在欧洲发现。最后一个进化枝（B）含有来自欧洲和马约特岛的菌株。焦磷酸测序数据证实了这些物种分布的地理差异，表明G。小孢子复合体显示生物地理结构。(c)2013 Elsevier GmbH. All rights reserved.
The Gomphonema parvulum complex includes species displaying considerable morphological variability and a wide geographical distribution. These characteristics make them difficult to identify by microscopy and raise the question of their taxonomic validity and of the possibility of biogeographical differentiation between them. In this context, we isolated 39 G. parvulum s.l. strains from rivers located in a tropical island (Mayotte) and in mainland Europe. By sequencing three DNA fragments (ITS, rbcL and cox1), four clades (A, B, C and D) were clearly identified, and an additional one (B') was distinguishable only on the rbcL sequence. The main four clades can be separated by their morphological criteria, in particular by the shape of the central area, but some overlaps were found between them. We therefore consider that the G. parvulum complex contains at least four semi-cryptic species corresponding to the four main clades. One of them (A) was found only on Mayotte, while two others (C and D) were found only in Europe. The last clade (B) contained strains from both Europe and Mayotte. Pyrosequencing data confirmed the geographical differences in the distribution of these species, suggesting that the G. parvulum complex displays biogeographic structure. (c) 2013 Elsevier GmbH. All rights reserved.