Lipoxygenase-dependent mechanisms in hypertension
Lipoxygenase-dependent mechanisms in hypertension
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DOI：
10.1081/ceh-100100071
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发表时间：
2000-02-01
影响因子：
12.3
通讯作者：
Nasjletti, A
中科院分区：
文献类型：
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作者：
DelliPizzi, A;Guan, H;Nasjletti, A
本研究旨在探讨脂氧合酶产物对主动脉缩窄诱导的高血压大鼠血管收缩和血压升高机制的作用。从高血压大鼠中提取的前列腺素的胞浆组分中，12-脂氧合酶蛋白与正常血压对照组相比增加。高血压大鼠的主动脉环表现出基础张力，在脂氧合酶抑制剂3，4-二羟基-α-氰基肉桂酸肉桂酯（CDC，10（-5）mol/L）和5，8，11-二十碳三炔酸（ETI，10（-5）mol/L）作用下，基础张力分别降低74+/-12和71+/-22%。CDC（8 mg/kg sc）不影响正常血压大鼠的血压，但使高血压大鼠的血压从182+/-6降至151+/-10 mm Hg。CDC的降血压作用在用吲哚美辛或抗5，6-二氢前列腺素I-2抗体预处理的高血压大鼠中减弱。这些数据表明，在主动脉缩窄诱导的高血压大鼠中，脂氧合酶衍生的产物对主动脉平滑肌基础张力和血压升高的机制有贡献。CDC的血管降压作用依赖于一种涉及血管舒张的肾上腺素的机制。
This study was designed to examine the contribution of lipoxygenase products to mechanisms of vascular contraction and elevated blood pressure in rats with aortic coarctation-induced hypertension. In cytosolic fractions of aortae taken from hypertensive rats, 12-lipoxygenase protein was increased as compared to normotensive controls. Aortic rings from hypertensive, but not from normotensive rats, exhibited a basal tone which was reduced 74+/-12 and 71+/-22%, respectively, by the lipoxygenase inhibitors cinnamyl-3,4-dihydroxy-alpha-cyanocinnamate (CDC,10(-5) mol/L) and 5,8,11-eicosatriynoic acid (ETI, 10(-5) mol/L). CDC (8mg/kg sc) did not affect the blood pressure of normotensive rats but decreased that of hypertensive rats from 182+/-6 to 151+/-10 mm Hg. The blood pressure lowering effect of CDC was blunted in hypertensive rats pretreated with indomethacin or antibodies against 5,6-dihydro-prostaglandin I-2. These data suggest contribution of lipoxygenase-derived products to mechanisms underlying aortic smooth muscle basal tone and elevated blood pressure in rats with aortic coarctation-induced hypertension. The vasodepressor effect of CDC depends on a mechanism involving vasodilatory prostaglandins.