Successful Release of Voriconazole and Flavonoids from MAPLE Deposited Bioactive Surfaces
Successful Release of Voriconazole and Flavonoids from MAPLE Deposited Bioactive Surfaces
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DOI：
10.3390/app9040786
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发表时间：
2019-02-02
影响因子：
2.7
通讯作者：
Chrisey, Douglas B.
中科院分区：
文献类型：
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作者：
Negut, Irina;Visan, Anita Ioana;Chrisey, Douglas B.
我们探索了利用基质辅助脉冲激光蒸发(Maple)方法制备的仿生薄膜释放以黄酮类化合物为代表的活性物质(二水合栎素和白藜芦醇)和嵌入在聚乙烯吡咯烷酮生物聚合物中的抗真菌化合物(两性霉素B和伏立康唑)的潜力，并用体外微生物学方法评价了薄膜组分的抗真菌活性。利用脉冲KrF*准分子激光光源制备了薄膜，并用原子力显微镜(AFM)和傅里叶变换红外光谱(FTIR)对薄膜进行了结构表征。当激光能量密度为80mJ/cm(2)时，获得了化学结构类似于滴铸结构的高质量薄膜。利用Maple技术将生物活性物质包含在聚合物薄膜中。采用改良的纸片扩散法对两株真菌菌株(白色念珠菌美国典型培养体系90028和近缘念珠菌美国典型培养体系22019)进行体外微生物检测，结果表明伏立康唑以活性形式从聚乙烯吡咯烷酮基质中释放出来。这项研究的结果表明，枫叶沉积的生物活性薄膜在设计组合产品和装置方面具有潜在的应用前景，例如药物输送装置和具有抗真菌活性的医疗装置表面。
We explored the potential of biomimetic thin films fabricated by means of matrix-assisted pulsed laser evaporation (MAPLE) for releasing combinations of active substances represented by flavonoids (quercetin dihydrate and resveratrol) and antifungal compounds (amphotericin B and voriconazole) embedded in a polyvinylpyrrolidone biopolymer; the antifungal activity of the film components was evaluated using in vitro microbiological assays. Thin films were deposited using a pulsed KrF* excimer laser source which were structurally characterized using atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). High-quality thin films with chemical structures similar to dropcast ones were created using an optimum laser fluence of similar to 80 mJ/cm(2). Bioactive substances were included within the polymer thin films using the MAPLE technique. The results of the in vitro microbiology assay, which utilized a modified disk diffusion approach and were performed using two fungal strains (Candida albicans American Type Culture Collection (ATCC) 90028 and Candida parapsilosis American Type Culture Collection (ATCC) 22019), revealed that voriconazole was released in an active form from the polyvinylpyrrolidone matrix. The results of this study show that the MAPLE-deposited bioactive thin films have a promising potential for use in designing combination products and devices, such as drug delivery devices, and medical device surfaces with antifungal activity.