β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitro
β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitro
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DOI：
10.1080/20002297.2019.1609839
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发表时间：
2019-01-01
影响因子：
4.5
通讯作者：
Nagamune, Hideaki
中科院分区：
文献类型：
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作者：
Tabata, Atsushi;Yamadab, Takuya;Nagamune, Hideaki
背景：咽峡炎链球菌亚种。心绞痛（SAA）是人类口腔中的机会致病菌之一。SAA的模式菌株NCTC 10713（T）在血琼脂平板上显示出明显的β-溶血，并且唯一的β-溶血因子显示出两个链球菌溶血素S（SLS）分子。SLS是由人类病原体S.在体外和体内，例如在小鼠感染模型中，其不仅对红细胞显示溶血活性，而且在细胞培养系中显示细胞毒性活性。然而，到目前为止还没有报道从β-溶血性SAA（β-SAA）产生的SLS的细胞毒性活性。目标与设计：在这项研究中，SLS依赖的细胞毒性的β-SAA菌株，包括基因修饰的菌株进行了研究。结果如下：在共培养条件下，产生SLS的β-SAA在人细胞培养系中显示出细胞毒性，并且发现这种细胞毒性是由分泌到细胞外环境中的SLS引起的。结论：本研究的结果表明，从β-SAA产生的SLS可能显示出与S.化脓性链球菌和产生SLS的β-SAA将被认为是“披着羊皮的狼”，β-溶血性心绞痛群链球菌的致病性将受到更多的关注。
Background: Streptococcus anginosus subsp. anginosus (SAA) is one of the opportunistic pathogens in humans that inhabits the oral cavity. The type strain of SAA, NCTC10713(T), showed clear beta-hemolysis on blood agar plates, and the sole beta-hemolytic factor revealed two streptolysin S (SLS) molecules. SLS is well known as the peptide hemolysin produced from the human pathogen S. pyogenes and shows not only hemolytic activity on erythrocytes but also cytotoxic activity in cell culture lines in vitro and in vivo, such as in a mouse infection model. However, no cytotoxic activity of SLS produced from beta-hemolytic SAA (beta-SAA) has been reported so far. Objective and Design: In this study, the SLS-dependent cytotoxicity of the beta-SAA strains including the genetically modified strains was investigated in vitro. Results: The SLS-producing beta-SAA showed cytotoxicity in human cell culture lines under the co-cultivation condition and it was found that this cytotoxicity was caused by the SLS secreted into the extracellular milieu. Conclusion: The results from this study suggest that the SLS produced from beta-SAA might indicate the cytotoxic potential similar to that of the SLS from S. pyogenes and the SLS-producing beta-SAA would be recognized as "a wolf in sheep's clothing" More attention will be paid to the pathogenicity of beta-hemolytic Anginosus group streptococci.