Surface plasmon resonance imaging (SPRI) system and real-time monitoring of DNA biochip for human genetic mutation diagnosis of DNA amplified samples
Surface plasmon resonance imaging (SPRI) system and real-time monitoring of DNA biochip for human genetic mutation diagnosis of DNA amplified samples
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DOI：
10.1016/j.snb.2006.01.023
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发表时间：
2006-12
影响因子：
8.4
通讯作者：
I. Mannelli;V. Courtois;P. Lecaruyer;G. Roger;M. Millot;M. Goossens;M. Canva
中科院分区：
文献类型：
--
作者：
I. Mannelli;V. Courtois;P. Lecaruyer;G. Roger;M. Millot;M. Goossens;M. Canva
基于核酸的生物芯片是一种很有前途的基因序列分析工具，尤其是突变检测。表面等离子体共振成像(SPRI)技术可以实时动态监测分子间的相互作用，例如DNA-DNA，而无需任何事先标记步骤。SPRI被应用于开发一种DNA传感器，用于检测与人类囊性纤维化有关的基因突变，特别是一些位于外显子10的基因突变，常见的三个碱基对缺失的ΔF508，以及几个单核苷酸多态(SNP)。SPRI系统使我们能够监测未标记的DNA靶标(短寡核苷酸或377pb的聚合酶链式反应产物)与固定在生物功能化表面的196个单链DNA探针矩阵的杂交动力学，并实时检测DNA片段中的突变。
Nucleic acid-based biochips represent a promising tool for gene sequence analysis, especially for mutation detection. Surface plasmon resonance imaging (SPRI) technique allows kinetic monitoring of molecular interaction, such as DNA–DNA, in real-time and without any prior labelling step. SPRI was applied to developing a DNA sensor for the detection of gene mutations accounting for human cystic fibrosis and specifically some of those localized in exon 10, with the common three-base-pair deletion ΔF508, alongside several single nucleotide polymorphisms (SNPs). The SPRI system enabled us to monitor the hybridisation kinetics of unlabelled DNA targets (short oligonucleotides or a 377pb PCR product) to a 196 spots matrix of ssDNA probes immobilised onto a bio-functionalised surface, and to detect in real-time the mutations in a DNA fragment.