Electroacupuncture alleviates ischaemic brain injury by regulating the miRNA-34/Wnt/autophagy axis
Electroacupuncture alleviates ischaemic brain injury by regulating the miRNA-34/Wnt/autophagy axis
复制标题
电针通过调节miRNA-34/Wnt/自噬轴减轻缺血性脑损伤
DOI：
10.1016/j.brainresbull.2021.02.002
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发表时间：
2021-02-19
影响因子：
3.8
通讯作者：
Zhou, Shuang
中科院分区：
文献类型：
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作者：
Cao, Siqiao;Yang, Yufang;Zhou, Shuang
电针作为一种现代针灸疗法，已广泛应用于缺血性脑损伤的治疗。然而，EA改善缺血性脑损伤的分子机制尚不清楚。本研究采用EA治疗大脑中动脉闭塞（MCAO）大鼠，通过检测脑梗死面积和神经细胞凋亡情况来确定EA的治疗效果，并通过高通量测序检测对照组、MCAO组和EA组MCAO之间的mirna表达差异。结果表明，EA治疗可减少神经细胞凋亡和缺血性梗死体积。三组间miR-34、miR-235、miR-275的差异均有统计学意义。此外，京都基因与基因组百科全书（KEGG）通路数据表明，Wnt通路可能在缺血性脑损伤和EA治疗中发挥重要作用。我们的数据表明，MCAO组miR-34明显升高，而EA治疗降低了miR-34的表达。WNT1是miR-34的靶标，并通过荧光素酶报告基因试验得到证实。先前的研究表明，在ea预处理的MCAO小鼠中，Wnt通路介导自噬。我们的数据进一步证实了MCAO后EA治疗也减轻了MCAO组的自噬。我们的研究结果表明，EA治疗通过miR-34/Wnt途径抑制自噬来减轻缺血性脑损伤。
Electroacupuncture (EA), a modern form of acupuncture therapy, has been widely used for the treatment of ischaemic brain injury. However, the molecular mechanism by which EA improves ischaemic brain injury remains unclear. In the current study, middle cerebral artery occlusion (MCAO) rats were treated with EA. The infarct volumes and apoptosis of neurocytes were assessed to determine the therapeutic effect of EA. The differentially expressed miRNAs between the control, MCAO and MCAO treated with EA groups were detected by high-throughput sequencing. The results indicated that EA treatment decreased neurocyte apoptosis and ischaemic infarct volume. Between the three groups, miR-34, miR-235 and miR-275 were found to be significantly different. Furthermore, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway data suggest that the Wnt pathway may play an important role in ischaemic brain injury and in the treatment of EA. Our data documented that miR-34 was obviously increased in the MCAO group, while EA treatment decreased miR-34 expression. WNT1 was the target of miR-34 and was confirmed by a luciferase reporter assay. A previous study suggested that the Wnt pathway mediates autophagy in EA-pretreated MCAO mice. Our data further confirmed that EA treatment after MCAO also alleviated autophagy in the MCAO group. Our results suggest that EA treatment alleviates ischaemic brain injury by inhibiting autophagy through the miR-34/Wnt pathway.